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@ARTICLE{Matsubara:56263,
author = {Matsubara, S. and Morosinotto, T. and Osmond, C. B. and
Bassi, R.},
title = {{S}hort- and long-term operation of the lutein-epoxide
cycle in light-harvesting antenna complexes},
journal = {Plant physiology},
volume = {144},
issn = {0032-0889},
address = {Rockville, Md.: Soc.},
publisher = {JSTOR},
reportid = {PreJuSER-56263},
pages = {926 - 941},
year = {2007},
note = {Record converted from VDB: 12.11.2012},
abstract = {The lutein-5,6-epoxide (Lx) cycle operates in some plants
between lutein (L) and its monoepoxide, Lx. Whereas recent
studies have established the photoprotective roles of the
analogous violaxanthin cycle, physiological functions of the
Lx cycle are still unknown. In this article, we investigated
the operation of the Lx cycle in light-harvesting antenna
complexes (Lhcs) of Inga sapindoides Willd, a tropical tree
legume accumulating substantial Lx in shade leaves, to
identify the xanthophyll-binding sites involved in short-
and long-term responses of the Lx cycle and to analyze the
effects on light-harvesting efficiency. In shade leaves, Lx
was converted into L upon light exposure, which then
replaced Lx in the peripheral V1 site in trimeric Lhcs and
the internal L2 site in both monomeric and trimeric Lhcs,
leading to xanthophyll composition resembling sun-type Lhcs.
Similar to the violaxanthin cycle, the Lx cycle was
operating in both photosystems, yet the light-induced Lx -->
L conversion was not reversible overnight. Interestingly,
the experiments using recombinant Lhcb5 reconstituted with
different Lx and/or L levels showed that reconstitution with
Lx results in a significantly higher fluorescence yield due
to higher energy transfer efficiencies among chlorophyll
(Chl) a molecules, as well as from xanthophylls to Chl a.
Furthermore, the spectroscopic analyses of photosystem
I-LHCI from I. sapindoides revealed prominent red-most Chl
forms, having the lowest energy level thus far reported for
higher plants, along with reduced energy transfer efficiency
from antenna pigments to Chl a. These results are discussed
in the context of photoacclimation and shade adaptation.},
keywords = {Adaptation, Physiological / Carotenoids: metabolism /
Chlorophyll: metabolism / Fabaceae: metabolism /
Light-Harvesting Protein Complexes: metabolism / Lutein:
analogs $\&$ derivatives / Lutein: metabolism / Photosystem
I Protein Complex: metabolism / Plant Leaves: metabolism /
Recombinant Proteins: metabolism / Spectrum Analysis /
Sunlight / Thylakoids: metabolism / Time Factors /
Light-Harvesting Protein Complexes (NLM Chemicals) /
Photosystem I Protein Complex (NLM Chemicals) / Recombinant
Proteins (NLM Chemicals) / lutein-5,6-epoxide (NLM
Chemicals) / Lutein (NLM Chemicals) / Chlorophyll (NLM
Chemicals) / Carotenoids (NLM Chemicals) / J (WoSType)},
cin = {ICG-3},
ddc = {580},
cid = {I:(DE-Juel1)ICG-3-20090406},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:17384157},
pmc = {pmc:PMC1914152},
UT = {WOS:000247075000036},
doi = {10.1104/pp.107.099077},
url = {https://juser.fz-juelich.de/record/56263},
}