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@ARTICLE{GarcaPlazaola:56789,
author = {García-Plazaola, J. I. and Matsubara, S. and Osmond, C.
B.},
title = {{T}he lutein epoxide cycle in higher plants: its
relationships to other xanthophyll cycles and possible
functions},
journal = {Functional plant biology},
volume = {34},
issn = {1445-4408},
address = {Collingwood, Victoria},
publisher = {CSIRO Publ.},
reportid = {PreJuSER-56789},
pages = {759 - 773},
year = {2007},
note = {Record converted from VDB: 12.11.2012},
abstract = {Several xanthophyll cycles have been described in
photosynthetic organisms. Among them, only two are present
in higher plants: the ubiquitous violaxanthin (V) cycle, and
the taxonomically restricted lutein epoxide (Lx) cycle,
whereas four cycles seem to occur in algae. Although V is
synthesised through the beta-branch of the carotenoid
biosynthetic pathway and Lx is the product of the
alpha-branch; both are co-located in the same sites of the
photosynthetic pigment- protein complexes isolated from
thylakoids. Both xanthophylls are also de-epoxidised upon
light exposure by the same enzyme, violaxanthin de-epoxidase
(VDE) leading to the formation of zeaxanthin (Z) and lutein
(L) at comparable rates. In contrast with VDE, the reverse
reaction presumably catalysed by zeaxanthin epoxidase (ZE),
is much slower (or even inactive) with L than with
antheraxanthin (A) or Z. Consequently many species lack Lx
altogether, and although the presence of Lx shows an
irregular taxonomical distribution in unrelated taxa, it has
a high fidelity at family level. In those plants which
accumulate Lx, variations in ZE activity in vivo mean that a
complete Lx-cycle occurs in some (with Lx pools being
restored overnight), whereas in others a truncated cycle is
observed in which VDE converts Lx into L, but regeneration
of Lx by ZE is extremely slow. Accumulation of Lx to high
concentrations is found most commonly in old leaves in
deeply shaded canopies, and the Lx cycle in these leaves is
usually truncated. This seemingly anomalous situation
presumably arises because ZE has a low but finite affinity
for L, and because deeply shaded leaves are not often
exposed to light intensities strong enough to activate VDE.
Notably, both in vitro and in vivo studies have recently
shown that accumulation of Lx can increase the light
harvesting efficiency in the antennae of PSII. We propose a
model for the truncated Lx cycle in strong light in which
VDE converts Lx to L which then occupies sites L2 and V1 in
the light-harvesting antenna complex of PSII (Lhcb),
displacing V and Z. There is correlative evidence that this
photoconverted L facilitates energy dissipation via
non-photochemical quenching and thereby converts a highly
efficient light harvesting system to an energy dissipating
system with improved capacity to engage photoprotection.
Operation of the alpha- and beta- xanthophyll cycles with
different L and Z epoxidation kinetics thus allows a
combination of rapidly and slowly reversible modulation of
light harvesting and photoprotection, with each cycle having
distinct effects. Based on the patchy taxonomical
distribution of Lx, we propose that the presence of Lx (and
the Lx cycle) could be the result of a recurrent mutation in
the epoxidase gene that increases its affinity for L, which
is conserved whenever it confers an evolutionary advantage.},
keywords = {J (WoSType)},
cin = {ICG-3},
ddc = {580},
cid = {I:(DE-Juel1)ICG-3-20090406},
pnm = {Terrestrische Umwelt},
pid = {G:(DE-Juel1)FUEK407},
shelfmark = {Plant Sciences},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000249104100001},
doi = {10.1071/FP07095},
url = {https://juser.fz-juelich.de/record/56789},
}