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@ARTICLE{Babay:57080,
      author       = {Babay, P. A. and Gettar, R. T. and Magallanes, J.F. and
                      Becquart, E.T. and Thiele, B. and Batistoni, D. A.},
      title        = {{C}olumn temperature as an active variable in the
                      isocratic, normal-phase high-performance liquid
                      chromatography separation of lipophilic metabolites of
                      nonylphenol ethoxylates},
      journal      = {Journal of chromatography / A},
      volume       = {1157},
      issn         = {0021-9673},
      address      = {New York, NY [u.a.]},
      publisher    = {Science Direct},
      reportid     = {PreJuSER-57080},
      pages        = {227 - 236},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Normal-phase separation of technical grade nonylphenol
                      (t-NP, about $90\%$ 4-nonylphenol), 4-nonylphenol
                      mono-ethoxylate (4-NP1EO) and 4-nonylphenol di-ethoxylate
                      (4-NP2EO) was assessed, with the inclusion of column
                      temperature as an active variable. The compound
                      2,4,6-trimethylphenol was evaluated for use as internal
                      standard. Isocratic elution with 2-propanol/hexanes mixtures
                      from an amino-silica column and spectrometric UV detection
                      at 277 nm were employed. Technical nonylphenol presented a
                      significant contribution from unknown substances that eluted
                      with retention times similar to that of 4-NP1EO. GC-MS
                      analysis of the unknowns allowed to identify them as isomers
                      of 2-NP. The response of the system to joint variations in
                      flow rate, eluent composition and column temperature was
                      investigated by means of Doehlert statistical experimental
                      design. A model for retention of the analytes as a function
                      of the experimental variables was proposed, and separation
                      selectivity was studied. Selection of the optimal working
                      zone was made through desirability function (D)
                      calculations. Potential co-elution of 2-NP isomers with
                      4-NP1EO was considered when optimizing the separation. The
                      occurrence of a restricted region of the experimental space
                      where baseline resolution of analytes, associated impurities
                      and internal standard results feasible (D not equal to 0) is
                      apparent.},
      keywords     = {Chromatography, High Pressure Liquid: instrumentation /
                      Ethylene Glycols: isolation $\&$ purification / Ethylene
                      Glycols: metabolism / Sensitivity and Specificity /
                      Spectrophotometry, Ultraviolet / Temperature / Ethylene
                      Glycols (NLM Chemicals) / terics (NLM Chemicals) / J
                      (WoSType)},
      cin          = {ICG-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICG-3-20090406},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Biochemical Research Methods / Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17540387},
      UT           = {WOS:000248107200028},
      doi          = {10.1016/j.chroma.2007.05.038},
      url          = {https://juser.fz-juelich.de/record/57080},
}