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000057094 084__ $$2WoS$$aInstruments & Instrumentation
000057094 084__ $$2WoS$$aPhysics, Applied
000057094 1001_ $$0P:(DE-Juel1)VDB57503$$aCesa, C. M.$$b0$$uFZJ
000057094 245__ $$aMicropatterned silicon elastomer for high resolution analysis of cell force patterns
000057094 260__ $$a[S.l.]$$bAmerican Institute of Physics$$c2007
000057094 300__ $$a034301
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000057094 440_0 $$05309$$aReview of Scientific Instruments$$v78$$x0034-6748$$y3
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000057094 520__ $$aCellular forces are closely related to many physiological processes, including cell migration, growth, division, and differentiation. Here, we describe newly developed techniques to measure these forces with high spatial resolution. Our approach is based on ultrasoft silicone elastomer films with a regular microstructure molded into the surface. Mechanical forces applied by living cells to such films result in elastomer deformation which can be quantified by video microscopy and digital image processing. From this deformation field forces can be calculated. Here we give detailed accounts of the following issues: (1) the preparation of silicon wafers as molds for the microstructures, (2) the fabrication of microstructured elastomer substrates, (3) the in-depth characterization of the mechanical properties of these elastomers, (4) the image processing algorithms for the extraction of cellular deformation fields, and (5) the generalized first moment tensor as a robust mathematical tool to characterize whole cell activity. We present prototype experiments on living myocytes as well as on cardiac fibroblasts and discuss the characteristics and performance of our force measurement technique.
000057094 536__ $$0G:(DE-Juel1)FUEK414$$2G:(DE-HGF)$$aKondensierte Materie$$cP54$$x0
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000057094 588__ $$aDataset connected to Web of Science, Pubmed
000057094 650_2 $$2MeSH$$aCell Adhesion
000057094 650_2 $$2MeSH$$aCell Physiological Phenomena
000057094 650_2 $$2MeSH$$aMechanics
000057094 650_2 $$2MeSH$$aMicroscopy: methods
000057094 650_2 $$2MeSH$$aSilicone Elastomers: chemistry
000057094 650_7 $$00$$2NLM Chemicals$$aSilicone Elastomers
000057094 650_7 $$2WoSType$$aJ
000057094 7001_ $$0P:(DE-Juel1)VDB8902$$aKirchgeßner, N.$$b1$$uFZJ
000057094 7001_ $$0P:(DE-Juel1)128707$$aMayer, D.$$b2$$uFZJ
000057094 7001_ $$0P:(DE-HGF)0$$aSchwarz, U. S.$$b3
000057094 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b4$$uFZJ
000057094 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b5$$uFZJ
000057094 773__ $$0PERI:(DE-600)1472905-2$$a10.1063/1.2712870$$gVol. 78, p. 034301$$p034301$$q78<034301$$tReview of scientific instruments$$v78$$x0034-6748$$y2007
000057094 8567_ $$uhttp://dx.doi.org/10.1063/1.2712870
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000057094 9131_ $$0G:(DE-Juel1)FUEK412$$bSchlüsseltechnologien$$kP42$$lGrundlagen für zukünftige Informationstechnologien (FIT)$$vGrundlagen für zukünftige Informationstechnologien$$x1
000057094 9141_ $$y2007
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