TY  - JOUR
AU  - Badura, A.
AU  - Esper, B.
AU  - Ataka, K.
AU  - Grunwald, A.
AU  - Wöll, C.
AU  - Kuhlmann, J.
AU  - Heberle, J.
AU  - Rögner, M.
TI  - Light-driven water splitting for (bio-)hydrogen production: photosystem 2 as the central part of a bioelectrochemical device
JO  - Photochemistry and photobiology
VL  - 82
SN  - 0031-8655
CY  - Malden, Mass.
PB  - Wiley-Blackwell
M1  - PreJuSER-57129
SP  - 1385 - 1390
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - To establish a semiartificial device for (bio-)hydrogen production utilizing photosynthetic water oxidation, we report on the immobilization of a Photosystem 2 on electrode surfaces. For this purpose, an isolated Photosystem 2 with a genetically introduced His tag from the cyanobacterium Thermosynechococcus elongatus was attached onto gold electrodes modified with thiolates bearing terminal Ni(II)-nitrilotriacetic acid groups. Surface enhanced infrared absorption spectroscopy showed the binding kinetics of Photosystem 2, whereas surface plasmon resonance measurements allowed the amount of protein adsorbed to be quantified. On the basis of these data, the surface coverage was calculated to be 0.29 pmol protein cm(-2), which is in agreement with the formation of a monomolecular film on the electrode surface. Upon illumination, the generation of a photocurrent was observed with current densities of up to 14 microA cm(-2) . This photocurrent is clearly dependent on light quality showing an action spectrum similar to an isolated Photosystem 2. The achieved current densities are equivalent to the highest reported oxygen evolution activities in solution under comparable conditions.
KW  - Electrochemistry: methods
KW  - Electrodes
KW  - Hydrogen: metabolism
KW  - Kinetics
KW  - Light
KW  - Photochemistry
KW  - Photosystem II Protein Complex: metabolism
KW  - Photosystem II Protein Complex: radiation effects
KW  - Synechococcus: metabolism
KW  - Synechococcus: radiation effects
KW  - Water: metabolism
KW  - Photosystem II Protein Complex (NLM Chemicals)
KW  - Hydrogen (NLM Chemicals)
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16898857
UR  - <Go to ISI:>//WOS:000241426600032
DO  - DOI:10.1562/2006-07-14-RC-969
UR  - https://juser.fz-juelich.de/record/57129
ER  -