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@ARTICLE{Mulkidjanian:57132,
author = {Mulkidjanian, A. Y. and Heberle, J. and Cherepanov, D. A.},
title = {{P}rotons @ interfaces: implications for biological energy
conversion},
journal = {Biochimica et biophysica acta / Bioenergetics},
volume = {1757},
issn = {0005-2728},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {PreJuSER-57132},
pages = {913 - 930},
year = {2006},
note = {Record converted from VDB: 12.11.2012},
abstract = {The review focuses on the anisotropy of proton transfer at
the surface of biological membranes. We consider (i) the
data from "pulsed" experiments, where light-triggered
enzymes capture or eject protons at the membrane surface,
(ii) the electrostatic properties of water at charged
interfaces, and (iii) the specific structural attributes of
proton-translocating enzymes. The pulsed experiments
revealed that proton exchange between the membrane surface
and the bulk aqueous phase takes as much as about 1 ms, but
could be accelerated by added mobile pH-buffers. Since the
accelerating capacity of the latter decreased with the
increase in their electric charge, it was concluded that the
membrane surface is separated from the bulk aqueous phase by
a barrier of electrostatic nature. The barrier could arise
owing to the water polarization at the negatively charged
membrane surface. The barrier height depends linearly on the
charge of penetrating ions; for protons, it has been
estimated as about 0.12 eV. While the proton exchange
between the surface and the bulk aqueous phase is retarded
by the interfacial barrier, the proton diffusion along the
membrane, between neighboring enzymes, takes only
microseconds. The proton spreading over the membrane is
facilitated by the hydrogen-bonded networks at the surface.
The membrane-buried layers of these networks can eventually
serve as a storage/buffer for protons (proton sponges). As
the proton equilibration between the surface and the bulk
aqueous phase is slower than the lateral proton diffusion
between the "sources" and "sinks", the proton activity at
the membrane surface, as sensed by the energy transducing
enzymes at steady state, might deviate from that measured in
the adjoining water phase. This trait should increase the
driving force for ATP synthesis, especially in the case of
alkaliphilic bacteria.},
keywords = {Biological Transport / Cations: chemistry / Electron
Transport Complex IV: chemistry / Energy Metabolism /
Kinetics / Membranes: physiology / Models, Biological /
Models, Molecular / Protein Conformation / Protons / Water:
chemistry / Cations (NLM Chemicals) / Protons (NLM
Chemicals) / Water (NLM Chemicals) / Electron Transport
Complex IV (NLM Chemicals) / J (WoSType)},
cin = {IBI-2},
ddc = {570},
cid = {I:(DE-Juel1)VDB58},
pnm = {Funktion und Dysfunktion des Nervensystems},
pid = {G:(DE-Juel1)FUEK409},
shelfmark = {Biochemistry $\&$ Molecular Biology / Biophysics},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:16624250},
UT = {WOS:000241481300005},
doi = {10.1016/j.bbabio.2006.02.015},
url = {https://juser.fz-juelich.de/record/57132},
}