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000057136 0247_ $$2DOI$$a10.1016/j.jneumeth.2006.01.006
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000057136 084__ $$2WoS$$aBiochemical Research Methods
000057136 084__ $$2WoS$$aNeurosciences
000057136 1001_ $$0P:(DE-HGF)0$$aLoesel, R.$$b0
000057136 245__ $$aA simple fluorescent double staining method for distinguishing neuronal from non-neuronal cells in the insect central nervous system
000057136 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2006
000057136 300__ $$a202 - 206
000057136 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000057136 440_0 $$09911$$aJournal of Neuroscience Methods$$v155$$x0165-0270$$y2
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000057136 520__ $$aBeing able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and Manduca sexta. Especially developmental or comparative studies often require an estimate of neuron numbers. Since neuronal and glial cell bodies are in many cases indiscernible in situ, a method to distinguish neurons from non-neuronal cells that works in any given species is wanting. Another application is cell culturing. Cultured cells usually change their outward shape dramatically after being isolated so that it is frequently impossible to tell neurons and glia apart. Here, we present a simple method that uses a commercially available antiserum directed against horseradish peroxidase, which specifically stains neurons but no other cell type in every insect species investigated. Counterstaining with DAPI, a fluorescent chromophore that binds to double-stranded DNA in the nuclei of all cells, yields the total number of cells in a given sample. Thus, double labeled cells can be identified as neurons, cells that carry only DAPI staining are non-neuronal.
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000057136 588__ $$aDataset connected to Web of Science, Pubmed
000057136 650_2 $$2MeSH$$aAnimals
000057136 650_2 $$2MeSH$$aCell Nucleus: metabolism
000057136 650_2 $$2MeSH$$aCells, Cultured
000057136 650_2 $$2MeSH$$aCentral Nervous System: cytology
000057136 650_2 $$2MeSH$$aDNA: metabolism
000057136 650_2 $$2MeSH$$aFluoresceins: metabolism
000057136 650_2 $$2MeSH$$aHorseradish Peroxidase: immunology
000057136 650_2 $$2MeSH$$aHorseradish Peroxidase: metabolism
000057136 650_2 $$2MeSH$$aImmune Sera: metabolism
000057136 650_2 $$2MeSH$$aIndoles: diagnostic use
000057136 650_2 $$2MeSH$$aInsects
000057136 650_2 $$2MeSH$$aNeuroglia: cytology
000057136 650_2 $$2MeSH$$aNeuroglia: metabolism
000057136 650_2 $$2MeSH$$aNeurons: cytology
000057136 650_2 $$2MeSH$$aNeurons: metabolism
000057136 650_7 $$00$$2NLM Chemicals$$aFluoresceins
000057136 650_7 $$00$$2NLM Chemicals$$aImmune Sera
000057136 650_7 $$00$$2NLM Chemicals$$aIndoles
000057136 650_7 $$047165-04-8$$2NLM Chemicals$$aDAPI
000057136 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
000057136 650_7 $$0EC 1.11.1.-$$2NLM Chemicals$$aHorseradish Peroxidase
000057136 650_7 $$2WoSType$$aJ
000057136 65320 $$2Author$$ahorseradish peroxidase
000057136 65320 $$2Author$$adAPI
000057136 65320 $$2Author$$aConfocal laser-scanning microscopy
000057136 65320 $$2Author$$aimnumocytochemistry
000057136 65320 $$2Author$$adouble fluorescent stainim
000057136 65320 $$2Author$$acell culture
000057136 65320 $$2Author$$ainsect brain
000057136 65320 $$2Author$$aGlia
000057136 7001_ $$0P:(DE-Juel1)VDB19870$$aWeigel, S.$$b1$$uFZJ
000057136 7001_ $$0P:(DE-HGF)0$$aBräunig, P.$$b2
000057136 773__ $$0PERI:(DE-600)1500499-5$$a10.1016/j.jneumeth.2006.01.006$$gVol. 155, p. 202 - 206$$p202 - 206$$q155<202 - 206$$tJournal of neuroscience methods$$v155$$x0165-0270$$y2006
000057136 8567_ $$uhttp://dx.doi.org/10.1016/j.jneumeth.2006.01.006
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000057136 9141_ $$aNachtrag$$y2006
000057136 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000057136 9201_ $$0I:(DE-Juel1)VDB42$$d31.12.2006$$gISG$$kISG-2$$lInstitut für Bio- und Chemosensoren$$x1
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