TY  - JOUR
AU  - Loesel, R.
AU  - Weigel, S.
AU  - Bräunig, P.
TI  - A simple fluorescent double staining method for distinguishing neuronal from non-neuronal cells in the insect central nervous system
JO  - Journal of neuroscience methods
VL  - 155
SN  - 0165-0270
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-57136
SP  - 202 - 206
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Being able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and Manduca sexta. Especially developmental or comparative studies often require an estimate of neuron numbers. Since neuronal and glial cell bodies are in many cases indiscernible in situ, a method to distinguish neurons from non-neuronal cells that works in any given species is wanting. Another application is cell culturing. Cultured cells usually change their outward shape dramatically after being isolated so that it is frequently impossible to tell neurons and glia apart. Here, we present a simple method that uses a commercially available antiserum directed against horseradish peroxidase, which specifically stains neurons but no other cell type in every insect species investigated. Counterstaining with DAPI, a fluorescent chromophore that binds to double-stranded DNA in the nuclei of all cells, yields the total number of cells in a given sample. Thus, double labeled cells can be identified as neurons, cells that carry only DAPI staining are non-neuronal.
KW  - Animals
KW  - Cell Nucleus: metabolism
KW  - Cells, Cultured
KW  - Central Nervous System: cytology
KW  - DNA: metabolism
KW  - Fluoresceins: metabolism
KW  - Horseradish Peroxidase: immunology
KW  - Horseradish Peroxidase: metabolism
KW  - Immune Sera: metabolism
KW  - Indoles: diagnostic use
KW  - Insects
KW  - Neuroglia: cytology
KW  - Neuroglia: metabolism
KW  - Neurons: cytology
KW  - Neurons: metabolism
KW  - Fluoresceins (NLM Chemicals)
KW  - Immune Sera (NLM Chemicals)
KW  - Indoles (NLM Chemicals)
KW  - DAPI (NLM Chemicals)
KW  - DNA (NLM Chemicals)
KW  - Horseradish Peroxidase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16481042
UR  - <Go to ISI:>//WOS:000241217200006
DO  - DOI:10.1016/j.jneumeth.2006.01.006
UR  - https://juser.fz-juelich.de/record/57136
ER  -