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@ARTICLE{Loesel:57136,
      author       = {Loesel, R. and Weigel, S. and Bräunig, P.},
      title        = {{A} simple fluorescent double staining method for
                      distinguishing neuronal from non-neuronal cells in the
                      insect central nervous system},
      journal      = {Journal of neuroscience methods},
      volume       = {155},
      issn         = {0165-0270},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {PreJuSER-57136},
      pages        = {202 - 206},
      year         = {2006},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Being able to discriminate between neurons and non-neuronal
                      cells such as glia and tracheal cells has been a major
                      problem in insect neuroscience, because glia-specific
                      antisera are available for only a small number of species
                      such as Drosophila melanogaster and Manduca sexta.
                      Especially developmental or comparative studies often
                      require an estimate of neuron numbers. Since neuronal and
                      glial cell bodies are in many cases indiscernible in situ, a
                      method to distinguish neurons from non-neuronal cells that
                      works in any given species is wanting. Another application
                      is cell culturing. Cultured cells usually change their
                      outward shape dramatically after being isolated so that it
                      is frequently impossible to tell neurons and glia apart.
                      Here, we present a simple method that uses a commercially
                      available antiserum directed against horseradish peroxidase,
                      which specifically stains neurons but no other cell type in
                      every insect species investigated. Counterstaining with
                      DAPI, a fluorescent chromophore that binds to
                      double-stranded DNA in the nuclei of all cells, yields the
                      total number of cells in a given sample. Thus, double
                      labeled cells can be identified as neurons, cells that carry
                      only DAPI staining are non-neuronal.},
      keywords     = {Animals / Cell Nucleus: metabolism / Cells, Cultured /
                      Central Nervous System: cytology / DNA: metabolism /
                      Fluoresceins: metabolism / Horseradish Peroxidase:
                      immunology / Horseradish Peroxidase: metabolism / Immune
                      Sera: metabolism / Indoles: diagnostic use / Insects /
                      Neuroglia: cytology / Neuroglia: metabolism / Neurons:
                      cytology / Neurons: metabolism / Fluoresceins (NLM
                      Chemicals) / Immune Sera (NLM Chemicals) / Indoles (NLM
                      Chemicals) / DAPI (NLM Chemicals) / DNA (NLM Chemicals) /
                      Horseradish Peroxidase (NLM Chemicals) / J (WoSType)},
      cin          = {ISG-2},
      ddc          = {610},
      cid          = {I:(DE-Juel1)VDB42},
      pnm          = {Grundlagen für zukünftige Informationstechnologien},
      pid          = {G:(DE-Juel1)FUEK412},
      shelfmark    = {Biochemical Research Methods / Neurosciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:16481042},
      UT           = {WOS:000241217200006},
      doi          = {10.1016/j.jneumeth.2006.01.006},
      url          = {https://juser.fz-juelich.de/record/57136},
}