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| Hauptseite > Publikationsdatenbank > A simple fluorescent double staining method for distinguishing neuronal from non-neuronal cells in the insect central nervous system > print |
| Hauptseite > Publikationsdatenbank > A simple fluorescent double staining method for distinguishing neuronal from non-neuronal cells in the insect central nervous system > print |
| 001 | 57136 | ||
| 005 | 20161225172153.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:16481042 |
| 024 | 7 | _ | |2 DOI |a 10.1016/j.jneumeth.2006.01.006 |
| 024 | 7 | _ | |2 WOS |a WOS:000241217200006 |
| 037 | _ | _ | |a PreJuSER-57136 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 610 |
| 084 | _ | _ | |2 WoS |a Biochemical Research Methods |
| 084 | _ | _ | |2 WoS |a Neurosciences |
| 100 | 1 | _ | |a Loesel, R. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a A simple fluorescent double staining method for distinguishing neuronal from non-neuronal cells in the insect central nervous system |
| 260 | _ | _ | |a Amsterdam [u.a.] |b Elsevier Science |c 2006 |
| 300 | _ | _ | |a 202 - 206 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Journal of Neuroscience Methods |x 0165-0270 |0 9911 |y 2 |v 155 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Being able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and Manduca sexta. Especially developmental or comparative studies often require an estimate of neuron numbers. Since neuronal and glial cell bodies are in many cases indiscernible in situ, a method to distinguish neurons from non-neuronal cells that works in any given species is wanting. Another application is cell culturing. Cultured cells usually change their outward shape dramatically after being isolated so that it is frequently impossible to tell neurons and glia apart. Here, we present a simple method that uses a commercially available antiserum directed against horseradish peroxidase, which specifically stains neurons but no other cell type in every insect species investigated. Counterstaining with DAPI, a fluorescent chromophore that binds to double-stranded DNA in the nuclei of all cells, yields the total number of cells in a given sample. Thus, double labeled cells can be identified as neurons, cells that carry only DAPI staining are non-neuronal. |
| 536 | _ | _ | |a Grundlagen für zukünftige Informationstechnologien |c P42 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK412 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Cell Nucleus: metabolism |
| 650 | _ | 2 | |2 MeSH |a Cells, Cultured |
| 650 | _ | 2 | |2 MeSH |a Central Nervous System: cytology |
| 650 | _ | 2 | |2 MeSH |a DNA: metabolism |
| 650 | _ | 2 | |2 MeSH |a Fluoresceins: metabolism |
| 650 | _ | 2 | |2 MeSH |a Horseradish Peroxidase: immunology |
| 650 | _ | 2 | |2 MeSH |a Horseradish Peroxidase: metabolism |
| 650 | _ | 2 | |2 MeSH |a Immune Sera: metabolism |
| 650 | _ | 2 | |2 MeSH |a Indoles: diagnostic use |
| 650 | _ | 2 | |2 MeSH |a Insects |
| 650 | _ | 2 | |2 MeSH |a Neuroglia: cytology |
| 650 | _ | 2 | |2 MeSH |a Neuroglia: metabolism |
| 650 | _ | 2 | |2 MeSH |a Neurons: cytology |
| 650 | _ | 2 | |2 MeSH |a Neurons: metabolism |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Fluoresceins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Immune Sera |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Indoles |
| 650 | _ | 7 | |0 47165-04-8 |2 NLM Chemicals |a DAPI |
| 650 | _ | 7 | |0 9007-49-2 |2 NLM Chemicals |a DNA |
| 650 | _ | 7 | |0 EC 1.11.1.- |2 NLM Chemicals |a Horseradish Peroxidase |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a horseradish peroxidase |
| 653 | 2 | 0 | |2 Author |a dAPI |
| 653 | 2 | 0 | |2 Author |a Confocal laser-scanning microscopy |
| 653 | 2 | 0 | |2 Author |a imnumocytochemistry |
| 653 | 2 | 0 | |2 Author |a double fluorescent stainim |
| 653 | 2 | 0 | |2 Author |a cell culture |
| 653 | 2 | 0 | |2 Author |a insect brain |
| 653 | 2 | 0 | |2 Author |a Glia |
| 700 | 1 | _ | |a Weigel, S. |b 1 |u FZJ |0 P:(DE-Juel1)VDB19870 |
| 700 | 1 | _ | |a Bräunig, P. |b 2 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |a 10.1016/j.jneumeth.2006.01.006 |g Vol. 155, p. 202 - 206 |p 202 - 206 |q 155<202 - 206 |0 PERI:(DE-600)1500499-5 |t Journal of neuroscience methods |v 155 |y 2006 |x 0165-0270 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/j.jneumeth.2006.01.006 |
| 909 | C | O | |o oai:juser.fz-juelich.de:57136 |p VDB |
| 913 | 1 | _ | |k P42 |v Grundlagen für zukünftige Informationstechnologien |l Grundlagen für zukünftige Informationstechnologien (FIT) |b Schlüsseltechnologien |0 G:(DE-Juel1)FUEK412 |x 0 |
| 914 | 1 | _ | |a Nachtrag |y 2006 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k ISG-2 |l Institut für Bio- und Chemosensoren |d 31.12.2006 |g ISG |0 I:(DE-Juel1)VDB42 |x 1 |
| 970 | _ | _ | |a VDB:(DE-Juel1)89880 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)IBN-2-20090406 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBN-2-20090406 |
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