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000057204 0247_ $$2DOI$$a10.1016/j.mimet.2006.08.004
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000057204 084__ $$2WoS$$aBiochemical Research Methods
000057204 084__ $$2WoS$$aMicrobiology
000057204 1001_ $$0P:(DE-HGF)0$$aMeyer, M. H. F.$$b0
000057204 245__ $$aMagnetic biosensor for the detection of Yersinia pestis
000057204 260__ $$aNew York, NY$$bElsevier$$c2007
000057204 300__ $$a218 - 224
000057204 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000057204 440_0 $$03539$$aJournal of Microbiological Methods$$v68$$x0167-7012
000057204 500__ $$aRecord converted from VDB: 12.11.2012
000057204 520__ $$aA novel type of magnetic-beads based magnetic biosensor is described for the detection of Yersinia pestis. Experiments were performed with the antigen fraction F1 of these bacteria. The magnetic sensor platform offers easy and reliable detection of Y. pestis by the use of magnetic beads for labelling and quantification in a single step due to their paramagnetic features. The system uses antiYPF1 antibodies as capture element on ABICAP columns as core element of the magnetic sensor. Several immobilization methods for antibodies on polyethylene were exploited. The established biosensor has a linear detection range of 25-300 ng/ml Y. pestis antigen F1 and a detection limit of 2.5 ng/ml in buffer and human blood serum. The presented sensor system is small, simple, portable and therefore usable as off-lab detection unit for medical and warfare analytes.
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000057204 650_2 $$2MeSH$$aAnimals
000057204 650_2 $$2MeSH$$aAntibodies, Bacterial: chemistry
000057204 650_2 $$2MeSH$$aAntigens, Bacterial: blood
000057204 650_2 $$2MeSH$$aBiosensing Techniques: instrumentation
000057204 650_2 $$2MeSH$$aBiosensing Techniques: methods
000057204 650_2 $$2MeSH$$aCell Line
000057204 650_2 $$2MeSH$$aImmunomagnetic Separation: instrumentation
000057204 650_2 $$2MeSH$$aImmunomagnetic Separation: methods
000057204 650_2 $$2MeSH$$aMice
000057204 650_2 $$2MeSH$$aPlague: microbiology
000057204 650_2 $$2MeSH$$aYersinia pestis: isolation & purification
000057204 650_7 $$00$$2NLM Chemicals$$aAntibodies, Bacterial
000057204 650_7 $$00$$2NLM Chemicals$$aAntigens, Bacterial
000057204 650_7 $$2WoSType$$aJ
000057204 65320 $$2Author$$aYersinia pestis
000057204 65320 $$2Author$$aantiYPF1
000057204 65320 $$2Author$$amagnetic detection
000057204 65320 $$2Author$$amagnetic beads
000057204 7001_ $$0P:(DE-HGF)0$$aStehr, M.$$b1
000057204 7001_ $$0P:(DE-HGF)0$$aBhuju, S.$$b2
000057204 7001_ $$0P:(DE-Juel1)128697$$aKrause, H.-J.$$b3$$uFZJ
000057204 7001_ $$0P:(DE-HGF)0$$aHartmann, M.$$b4
000057204 7001_ $$0P:(DE-HGF)0$$aMiethe, P.$$b5
000057204 7001_ $$0P:(DE-HGF)0$$aSingh, M.$$b6
000057204 7001_ $$0P:(DE-HGF)0$$aKeusgen, M.$$b7
000057204 773__ $$0PERI:(DE-600)1483012-7$$a10.1016/j.mimet.2006.08.004$$gVol. 68, p. 218 - 224$$p218 - 224$$q68<218 - 224$$tJournal of microbiological methods$$v68$$x0167-7012$$y2007
000057204 8567_ $$uhttp://dx.doi.org/10.1016/j.mimet.2006.08.004
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000057204 9141_ $$y2007
000057204 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
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000057204 9201_ $$0I:(DE-Juel1)IBN-2-20090406$$d31.12.2010$$gIBN$$kIBN-2$$lBioelektronik$$x0
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