TY  - JOUR
AU  - Meyer, M. H. F.
AU  - Stehr, M.
AU  - Bhuju, S.
AU  - Krause, H.-J.
AU  - Hartmann, M.
AU  - Miethe, P.
AU  - Singh, M.
AU  - Keusgen, M.
TI  - Magnetic biosensor for the detection of Yersinia pestis
JO  - Journal of microbiological methods
VL  - 68
SN  - 0167-7012
CY  - New York, NY
PB  - Elsevier
M1  - PreJuSER-57204
SP  - 218 - 224
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - A novel type of magnetic-beads based magnetic biosensor is described for the detection of Yersinia pestis. Experiments were performed with the antigen fraction F1 of these bacteria. The magnetic sensor platform offers easy and reliable detection of Y. pestis by the use of magnetic beads for labelling and quantification in a single step due to their paramagnetic features. The system uses antiYPF1 antibodies as capture element on ABICAP columns as core element of the magnetic sensor. Several immobilization methods for antibodies on polyethylene were exploited. The established biosensor has a linear detection range of 25-300 ng/ml Y. pestis antigen F1 and a detection limit of 2.5 ng/ml in buffer and human blood serum. The presented sensor system is small, simple, portable and therefore usable as off-lab detection unit for medical and warfare analytes.
KW  - Animals
KW  - Antibodies, Bacterial: chemistry
KW  - Antigens, Bacterial: blood
KW  - Biosensing Techniques: instrumentation
KW  - Biosensing Techniques: methods
KW  - Cell Line
KW  - Immunomagnetic Separation: instrumentation
KW  - Immunomagnetic Separation: methods
KW  - Mice
KW  - Plague: microbiology
KW  - Yersinia pestis: isolation & purification
KW  - Antibodies, Bacterial (NLM Chemicals)
KW  - Antigens, Bacterial (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17011649
UR  - <Go to ISI:>//WOS:000244382400003
DO  - DOI:10.1016/j.mimet.2006.08.004
UR  - https://juser.fz-juelich.de/record/57204
ER  -