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@ARTICLE{Meyer:57204,
      author       = {Meyer, M. H. F. and Stehr, M. and Bhuju, S. and Krause,
                      H.-J. and Hartmann, M. and Miethe, P. and Singh, M. and
                      Keusgen, M.},
      title        = {{M}agnetic biosensor for the detection of {Y}ersinia
                      pestis},
      journal      = {Journal of microbiological methods},
      volume       = {68},
      issn         = {0167-7012},
      address      = {New York, NY},
      publisher    = {Elsevier},
      reportid     = {PreJuSER-57204},
      pages        = {218 - 224},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {A novel type of magnetic-beads based magnetic biosensor is
                      described for the detection of Yersinia pestis. Experiments
                      were performed with the antigen fraction F1 of these
                      bacteria. The magnetic sensor platform offers easy and
                      reliable detection of Y. pestis by the use of magnetic beads
                      for labelling and quantification in a single step due to
                      their paramagnetic features. The system uses antiYPF1
                      antibodies as capture element on ABICAP columns as core
                      element of the magnetic sensor. Several immobilization
                      methods for antibodies on polyethylene were exploited. The
                      established biosensor has a linear detection range of 25-300
                      ng/ml Y. pestis antigen F1 and a detection limit of 2.5
                      ng/ml in buffer and human blood serum. The presented sensor
                      system is small, simple, portable and therefore usable as
                      off-lab detection unit for medical and warfare analytes.},
      keywords     = {Animals / Antibodies, Bacterial: chemistry / Antigens,
                      Bacterial: blood / Biosensing Techniques: instrumentation /
                      Biosensing Techniques: methods / Cell Line / Immunomagnetic
                      Separation: instrumentation / Immunomagnetic Separation:
                      methods / Mice / Plague: microbiology / Yersinia pestis:
                      isolation $\&$ purification / Antibodies, Bacterial (NLM
                      Chemicals) / Antigens, Bacterial (NLM Chemicals) / J
                      (WoSType)},
      cin          = {CNI / IBN-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB381 / I:(DE-Juel1)IBN-2-20090406},
      pnm          = {Grundlagen für zukünftige Informationstechnologien},
      pid          = {G:(DE-Juel1)FUEK412},
      shelfmark    = {Biochemical Research Methods / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17011649},
      UT           = {WOS:000244382400003},
      doi          = {10.1016/j.mimet.2006.08.004},
      url          = {https://juser.fz-juelich.de/record/57204},
}