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000057344 0247_ $$2pmid$$apmid:16768508
000057344 0247_ $$2DOI$$a10.1021/la053310+
000057344 0247_ $$2WOS$$aWOS:000238217000044
000057344 037__ $$aPreJuSER-57344
000057344 041__ $$aeng
000057344 082__ $$a670
000057344 084__ $$2WoS$$aChemistry, Multidisciplinary
000057344 084__ $$2WoS$$aChemistry, Physical
000057344 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000057344 1001_ $$0P:(DE-Juel1)VDB57655$$aSengupta, K.$$b0$$uFZJ
000057344 245__ $$aCoupling artificial actin cortices to bio-functionalised lipid monolayers
000057344 260__ $$aWashington, DC$$bACS Publ.$$c2006
000057344 300__ $$a5776 - 5785
000057344 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000057344 440_0 $$04081$$aLangmuir$$v22$$x0743-7463$$y13
000057344 500__ $$aRecord converted from VDB: 12.11.2012
000057344 520__ $$aWe report the assembly of protein supramolecular structures at an air-water interface and coupling of artificial actin cortices to such structures. The coupling strategies adopted include electrostatic binding of actin to monolayers doped with lipids, exposing positively charged poly(ethylene glycol) headgroups; binding of biotinylated actin to lipids carrying biotin headgroups through avidin; binding of actin to membranes through biotinylated hisactophilin (a cellular actin-membrane coupler) using an avidin-biotin linkage; and coupling of actin to membranes carrying chelating lipids through a 15-nm-diameter protein capsid (bacterial lumazine synthase or LuSy) exhibiting histidine tags (which bind both to actin and to the chelating lipid). The distribution of the proteins in a direction normal to the interface was measured by neutron reflectivity under different conditions of pH and ionic strength. In the case of the first three binding methods, the thickness of the actin film was found to correspond to a single actin filament. Multilayers of actin could be formed only by using the multifunctional LuSy couplers that exhibit 60 hexahistidine tags and can thus act as actin cross-linkers. The LuSy-mediated binding can be reversibly switched by pH variations.
000057344 536__ $$0G:(DE-Juel1)FUEK414$$2G:(DE-HGF)$$aKondensierte Materie$$cP54$$x0
000057344 588__ $$aDataset connected to Web of Science, Pubmed
000057344 650_2 $$2MeSH$$aActins: chemistry
000057344 650_2 $$2MeSH$$aAnimals
000057344 650_2 $$2MeSH$$aBinding Sites
000057344 650_2 $$2MeSH$$aLipid Bilayers: chemistry
000057344 650_2 $$2MeSH$$aLipids: chemistry
000057344 650_2 $$2MeSH$$aMicrofilament Proteins: chemistry
000057344 650_2 $$2MeSH$$aModels, Molecular
000057344 650_2 $$2MeSH$$aMultienzyme Complexes: chemistry
000057344 650_2 $$2MeSH$$aMultiprotein Complexes: chemistry
000057344 650_2 $$2MeSH$$aNeutrons
000057344 650_2 $$2MeSH$$aProtein Binding
000057344 650_2 $$2MeSH$$aProtozoan Proteins: chemistry
000057344 650_2 $$2MeSH$$aStatic Electricity
000057344 650_2 $$2MeSH$$aSurface Properties
000057344 650_7 $$00$$2NLM Chemicals$$aActins
000057344 650_7 $$00$$2NLM Chemicals$$aLipid Bilayers
000057344 650_7 $$00$$2NLM Chemicals$$aLipids
000057344 650_7 $$00$$2NLM Chemicals$$aMicrofilament Proteins
000057344 650_7 $$00$$2NLM Chemicals$$aMultienzyme Complexes
000057344 650_7 $$00$$2NLM Chemicals$$aMultiprotein Complexes
000057344 650_7 $$00$$2NLM Chemicals$$aProtozoan Proteins
000057344 650_7 $$0122962-20-3$$2NLM Chemicals$$ahisactophilin protein, Protozoan
000057344 650_7 $$089287-46-7$$2NLM Chemicals$$a6,7-dimethyl-8-ribityllumazine synthase
000057344 650_7 $$2WoSType$$aJ
000057344 7001_ $$0P:(DE-HGF)0$$aLimozin, L.$$b1
000057344 7001_ $$0P:(DE-HGF)0$$aTristl, M.$$b2
000057344 7001_ $$0P:(DE-HGF)0$$aHaase, I.$$b3
000057344 7001_ $$0P:(DE-HGF)0$$aFischer, M.$$b4
000057344 7001_ $$0P:(DE-HGF)0$$aSackmann, E.$$b5
000057344 773__ $$0PERI:(DE-600)2005937-1$$a10.1021/la053310+$$gVol. 22, p. 5776 - 5785$$p5776 - 5785$$q22<5776 - 5785$$tLangmuir$$v22$$x0743-7463$$y2006
000057344 8567_ $$uhttp://dx.doi.org/10.1021/la053310+
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000057344 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt   bis 2009
000057344 9141_ $$aNachtrag$$y2006
000057344 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000057344 9201_ $$0I:(DE-Juel1)VDB421$$d31.12.2006$$gISG$$kISG-4$$lBiologische Schichten$$x0
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