TY  - JOUR
AU  - Sengupta, K.
AU  - Limozin, L.
AU  - Tristl, M.
AU  - Haase, I.
AU  - Fischer, M.
AU  - Sackmann, E.
TI  - Coupling artificial actin cortices to bio-functionalised lipid monolayers
JO  - Langmuir
VL  - 22
SN  - 0743-7463
CY  - Washington, DC
PB  - ACS Publ.
M1  - PreJuSER-57344
SP  - 5776 - 5785
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - We report the assembly of protein supramolecular structures at an air-water interface and coupling of artificial actin cortices to such structures. The coupling strategies adopted include electrostatic binding of actin to monolayers doped with lipids, exposing positively charged poly(ethylene glycol) headgroups; binding of biotinylated actin to lipids carrying biotin headgroups through avidin; binding of actin to membranes through biotinylated hisactophilin (a cellular actin-membrane coupler) using an avidin-biotin linkage; and coupling of actin to membranes carrying chelating lipids through a 15-nm-diameter protein capsid (bacterial lumazine synthase or LuSy) exhibiting histidine tags (which bind both to actin and to the chelating lipid). The distribution of the proteins in a direction normal to the interface was measured by neutron reflectivity under different conditions of pH and ionic strength. In the case of the first three binding methods, the thickness of the actin film was found to correspond to a single actin filament. Multilayers of actin could be formed only by using the multifunctional LuSy couplers that exhibit 60 hexahistidine tags and can thus act as actin cross-linkers. The LuSy-mediated binding can be reversibly switched by pH variations.
KW  - Actins: chemistry
KW  - Animals
KW  - Binding Sites
KW  - Lipid Bilayers: chemistry
KW  - Lipids: chemistry
KW  - Microfilament Proteins: chemistry
KW  - Models, Molecular
KW  - Multienzyme Complexes: chemistry
KW  - Multiprotein Complexes: chemistry
KW  - Neutrons
KW  - Protein Binding
KW  - Protozoan Proteins: chemistry
KW  - Static Electricity
KW  - Surface Properties
KW  - Actins (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Lipids (NLM Chemicals)
KW  - Microfilament Proteins (NLM Chemicals)
KW  - Multienzyme Complexes (NLM Chemicals)
KW  - Multiprotein Complexes (NLM Chemicals)
KW  - Protozoan Proteins (NLM Chemicals)
KW  - hisactophilin protein, Protozoan (NLM Chemicals)
KW  - 6,7-dimethyl-8-ribityllumazine synthase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16768508
UR  - <Go to ISI:>//WOS:000238217000044
DO  - DOI:10.1021/la053310+
UR  - https://juser.fz-juelich.de/record/57344
ER  -