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000057377 0247_ $$2pmid$$apmid:17368449
000057377 0247_ $$2DOI$$a10.1016/j.febslet.2007.03.005
000057377 0247_ $$2WOS$$aWOS:000245913300036
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000057377 041__ $$aeng
000057377 082__ $$a570
000057377 084__ $$2WoS$$aBiochemistry & Molecular Biology
000057377 084__ $$2WoS$$aBiophysics
000057377 084__ $$2WoS$$aCell Biology
000057377 1001_ $$0P:(DE-HGF)0$$aMennes, N.$$b0
000057377 245__ $$aExpression of the halobacterial transducer protein HtrII from Natronomonas pharaonis in Escherichia coli
000057377 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2007
000057377 300__ $$a1487 - 1494
000057377 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000057377 440_0 $$02052$$aFEBS Letters$$v581$$x0014-5793$$y7
000057377 500__ $$aRecord converted from VDB: 12.11.2012
000057377 520__ $$aArchaeal phototaxis is mediated by sensory rhodopsins which form complexes with their cognate transducers. Whereas the receptors sensory rhodopsin I and sensory rhodopsin II (SRII) have been expressed in Escherichia coli (E. coli) only shortened fragments of HtrII from Natronomonas pharaonis (NpHtrII) are available. Here we describe the heterologous expression of full length NpHtrII which was achieved in yields of up to 0.9 mg per litre cell culture. Gel filtration analysis reveals the tendency of the transducer to form dimers and higher-order oligomers which was also observed when complexed to NpSRII. A circular dichroism (CD) spectrum of NpHtrII is comparable to those obtained for the E. coli chemoreceptors indicating a similar folding with predominantly alpha-helical structure. NpHtrII dissociates from the NpSRII/HtrII complex with an apparent K(D) of about 0.6 microM. Photocycle kinetics of the complex is comparable to that obtained for NpSRII in complex with a truncated transducer with slight differences in the M-decay. The data indicate that the heterologously expressed NpHtrII adopt a native like structure, providing the means for elucidating transmembrane signal transduction and activation of microbial signalling cascades.
000057377 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000057377 588__ $$aDataset connected to Web of Science, Pubmed
000057377 650_2 $$2MeSH$$aArchaeal Proteins: biosynthesis
000057377 650_2 $$2MeSH$$aArchaeal Proteins: chemistry
000057377 650_2 $$2MeSH$$aArchaeal Proteins: isolation & purification
000057377 650_2 $$2MeSH$$aChromatography, Gel
000057377 650_2 $$2MeSH$$aCircular Dichroism
000057377 650_2 $$2MeSH$$aCloning, Molecular
000057377 650_2 $$2MeSH$$aDimerization
000057377 650_2 $$2MeSH$$aEscherichia coli: genetics
000057377 650_2 $$2MeSH$$aLasers
000057377 650_2 $$2MeSH$$aPhotolysis
000057377 650_2 $$2MeSH$$aProtein Structure, Secondary
000057377 650_2 $$2MeSH$$aRecombinant Proteins: biosynthesis
000057377 650_2 $$2MeSH$$aRecombinant Proteins: chemistry
000057377 650_2 $$2MeSH$$aRecombinant Proteins: isolation & purification
000057377 650_7 $$00$$2NLM Chemicals$$aArchaeal Proteins
000057377 650_7 $$00$$2NLM Chemicals$$aHtrII protein, Natronobacterium pharaonis
000057377 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000057377 650_7 $$2WoSType$$aJ
000057377 65320 $$2Author$$asensory rhodopsin
000057377 65320 $$2Author$$asignal transduction
000057377 65320 $$2Author$$aphototaxis
000057377 65320 $$2Author$$achemotaxis
000057377 7001_ $$0P:(DE-HGF)0$$aKlare, J. P.$$b1
000057377 7001_ $$0P:(DE-HGF)0$$aChizhov, I.$$b2
000057377 7001_ $$0P:(DE-HGF)0$$aSeidel, R.$$b3
000057377 7001_ $$0P:(DE-Juel1)VDB1421$$aSchlesinger, R.$$b4$$uFZJ
000057377 7001_ $$0P:(DE-HGF)0$$aEngelhard, M.$$b5
000057377 773__ $$0PERI:(DE-600)1460391-3$$a10.1016/j.febslet.2007.03.005$$gVol. 581, p. 1487 - 1494$$p1487 - 1494$$q581<1487 - 1494$$tFEBS letters$$v581$$x0014-5793$$y2007
000057377 8567_ $$uhttp://dx.doi.org/10.1016/j.febslet.2007.03.005
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000057377 9141_ $$y2007
000057377 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000057377 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
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000057377 980__ $$aI:(DE-Juel1)ICS-6-20110106
000057377 981__ $$aI:(DE-Juel1)IBI-7-20200312
000057377 981__ $$aI:(DE-Juel1)ISB-2-20090406
000057377 981__ $$aI:(DE-Juel1)ICS-6-20110106