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@ARTICLE{Halder:57645,
author = {Halder, S. and Gerber, P. and Schneller, T. and Waser, R.},
title = {{S}tructural, dielectric and electrochemical study of
{H}f-substituted {B}a{T}i{O}3 thin films fabricated by
{CSD}},
journal = {Applied physics / A},
volume = {83},
issn = {0947-8396},
address = {Berlin},
publisher = {Springer},
reportid = {PreJuSER-57645},
pages = {285 - 288},
year = {2006},
note = {Record converted from VDB: 12.11.2012},
abstract = {The immune environment of human soft-tissue injury is
unstudied. We studied fracture soft-tissue hematomas (FxSTH)
in 56 patients with high-energy bony fractures. FxSTH serum
and mononuclear cells (MNC) as well as fracture patient
plasma and blood MNC were studied. Twenty healthy controls
donated plasma and MNC. Soluble tumor necrosis factor
(TNF)-alpha, interleukin (IL-1 beta, IL-2, 6, 8, 10, 12, and
interferon-gamma were studied by enzyme linked immunosorbent
assay. Cells were studied by flow cytometry after
cell-membrane stains for CD-14, TNF-alpha (mTNF), and human
leukocyte antigen-DR, or intracellular stains for TNF
(icTNF) and IL-10. Thirty-six patients with Injury Severity
Score < 15 were analyzed further to evaluate the effects of
isolated fracture on systemic immunity. Cytokines were
rarely detectable in control plasma. TNF-alpha, IL-1 beta,
IL-2, and interferon-gamma were rarely found in FxSTH serum
or fracture patient plasma. All FxSTH sera were rich in
IL-6, peaking before 48 hours (12,538 +/- 4,153 vs. 3,494
+/- 909 pg/mL, p = 0.02, U test). In Injury Severity Score <
15, IL-6 was not detectable in most early fracture patient
plasma, but rose after 48 hours (p = 0.028). FxSTH serum
IL-8 peaked after 48 hours (440 +/- 289 vs. 4,542 +/- 1,219
pg/mL, p = 0.006) and circulating IL-8 appeared after 72
hours. IL-6 and IL-8 showed gradients from FxSTH serum to
paired PtS (p < 0.05, Wilcoxon). IL-10 was abundant (884 +/-
229 pg/mL) in FxSTH serum < 24 hours old. FxSTH serum IL-12
peaked late (3,323 +/- 799 pg/mL, day 4-7) then fell (p <
0.001, analysis of variance). Only IL-12 was higher in
fracture patient plasma (1,279 +/- 602 pg/mL) than FxSTH
serum (591 +/- 327 pg/mL) during the first 48 hours (p =
0.032, U test). On flow cytometry, control monocytes
expressed 201 +/- 31 mTNF sites/cell, but icTNF was absent.
mTNF was up-regulated after injury more in FxSTH monocytes
(3,202 +/- 870 sites/cell) than peripheral blood monocytes
(584 +/- 186 sites/cell) (p < 0.05 vs. peripheral blood
monocytes by Wilcoxon, p < 0.001 vs. control monocytes by U
test). Intracellular IL-10 was abundant in all MNC, but
varied widely after injury. Fracture and peripheral blood
monocytes expressed far less human leukocyte antigen-DR than
control monocytes. Fractures create an inflammatory local
environment. Proximal mediators are cell-associated and
relatively confined to the wound, but soluble IL-6, IL-8,
and IL-10 are abundant and probably exported. Systemic MNC
have complex responses to local injuries. These may reflect
the combined impact of multiple soluble cytokines initially
generated within the wound. FxSTH appear to be a potentially
important source of immunomodulatory cytokines in trauma.},
keywords = {Adolescent / Adult / Aged / Aged, 80 and over / Analysis of
Variance / Case-Control Studies / Cytokines: blood / Female
/ Fractures, Bone: complications / Fractures, Bone:
immunology / HLA-DR Antigens: blood / Hematoma:
complications / Hematoma: immunology / Humans / Immunity,
Cellular / Male / Middle Aged / Monocytes: immunology / Soft
Tissue Injuries: complications / Soft Tissue Injuries:
immunology / Time Factors / Cytokines (NLM Chemicals) /
HLA-DR Antigens (NLM Chemicals) / J (WoSType)},
cin = {IFF-IEM / CNI / JARA-FIT},
ddc = {530},
cid = {I:(DE-Juel1)VDB321 / I:(DE-Juel1)VDB381 /
$I:(DE-82)080009_20140620$},
pnm = {Kondensierte Materie},
pid = {G:(DE-Juel1)FUEK414},
shelfmark = {Materials Science, Multidisciplinary / Physics, Applied},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:9191672},
UT = {WOS:000236641800022},
doi = {10.1007/s00339-006-3494-3},
url = {https://juser.fz-juelich.de/record/57645},
}