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@ARTICLE{Dertinger:58183,
      author       = {Dertinger, T. and Pacheco, V. and von der Hocht, I. and
                      Hartmann, R. and Gregor, I. and Enderlein, J.},
      title        = {{T}wo focus fluorescence correlation spectroscopy: {A} new
                      tool for accurate and absolute diffusion measurements},
      journal      = {ChemPhysChem},
      volume       = {8},
      issn         = {1439-4235},
      address      = {Weinheim},
      publisher    = {Wiley-VCH Verl.},
      reportid     = {PreJuSER-58183},
      pages        = {433 - 443},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {We present a new method to measure absolute diffusion
                      coefficients at nanomolar concentrations with high
                      precision. Based on a modified fluorescence correlation
                      spectroscopy (FCS)-setup, this method is improved by
                      introducing an external ruler for measuring the diffusion
                      time by generating two laterally shifted and overlapping
                      laser foci at a fixed and known distance. Data fitting is
                      facilitated by a new two-parameter model to describe the
                      molecule detection function (MDF). We present a recorded MDF
                      and show the excellent agreement with the fitting model. We
                      measure the diffusion coefficient of the red fluorescent dye
                      Atto655 under various conditions and compare these values
                      with a value achieved by gradient pulsed field NMR (GPF
                      NMR). From these measurements we conclude, that the new
                      measurement scheme is robust against optical and
                      photophysical artefacts which are inherent to standard FCS.
                      With two-focus-FCS, the diffusion coefficient of 4.26 x
                      10(-6) cm2s(-1) for Atto655 in water at 25 degrees C
                      compares well with the GPF NMR value of 4.28 x 10(-6)
                      cm2s(-1).},
      keywords     = {Diffusion / Sensitivity and Specificity / Solutions /
                      Spectrometry, Fluorescence: methods / Viscosity / Solutions
                      (NLM Chemicals) / J (WoSType)},
      cin          = {INB-1 / INB-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB804 / I:(DE-Juel1)VDB805},
      pnm          = {Funktion und Dysfunktion des Nervensystems},
      pid          = {G:(DE-Juel1)FUEK409},
      shelfmark    = {Chemistry, Physical / Physics, Atomic, Molecular $\&$
                      Chemical},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17269116},
      UT           = {WOS:000244496200011},
      doi          = {10.1002/cphc.200600638},
      url          = {https://juser.fz-juelich.de/record/58183},
}