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000058486 0247_ $$2pmid$$apmid:17631530
000058486 0247_ $$2pmc$$apmc:PMC2025668
000058486 0247_ $$2DOI$$a10.1529/biophysj.107.105544
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000058486 084__ $$2WoS$$aBiophysics
000058486 1001_ $$0P:(DE-HGF)0$$aLimozin, L.$$b0
000058486 245__ $$aModulation of Vesicle Adhesion and Spreading Kinetics by Hyaluronan Cushions
000058486 260__ $$aNew York, NY$$bRockefeller Univ. Press$$c2007
000058486 300__ $$a3300 - 3313
000058486 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000058486 440_0 $$0882$$aBiophysical Journal$$v93$$x0006-3495
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000058486 520__ $$aThe adhesion of giant unilamellar phospholipid vesicles to planar substrates coated with extracellular matrix mimetic cushions of hyaluronan is studied using quantitative reflection interference contrast microscopy. The absolute height of the vesicle membrane at the vicinity of the substrate is measured by considering, for the first time, the refractive indices of the reflecting media. The thickness of the cushion is varied in the range of approximately 50-100 nm, by designing various coupling strategies. On bare protein-coated substrates, the vesicles spread fast (0.5 s) and form a uniform adhesion disk, with the average membrane height approximately 4 nm. On thick hyaluronan cushions (>80 nm), the membrane height is approximately the same as the thickness of the cushion, implying that the vesicle lies on top of the cushion. On a thin and inhomogeneous hyaluronan cushion, the adhesion is modified but not prevented. The spreading is slow ( approximately 20 s) compared to the no-cushion case. The average membrane height is approximately 10 nm and the adhesion disk is studded with blisterlike structures. Observations with fluorescent hyaluronan indicate that the polymer is compressed under, rather than expelled from, the adhesion disk. The adhesion energy density is approximately threefold higher in the no-cushion case (1.2 microJ/m(2)) as compared to the thin-cushion case (0.54 microJ/m(2)). In the thin-cushion case, the presence of short ( approximately 4 nm) glyco-polymers on the vesicles results in a hitherto unreported stable partial adhesion state--the membrane height ranges from zero to approximately 250 nm. The minimal model system presented here mimics in vitro the hyaluronan-modulated early stages of cell adhesion, and demonstrates that the presence of a polymer cushion influences both the final equilibrium adhesion-state and the spreading kinetics.
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000058486 650_2 $$2MeSH$$aAvidin: chemistry
000058486 650_2 $$2MeSH$$aHyaluronic Acid: chemistry
000058486 650_2 $$2MeSH$$aHyaluronic Acid: physiology
000058486 650_2 $$2MeSH$$aKinetics
000058486 650_2 $$2MeSH$$aMicroscopy, Interference
000058486 650_2 $$2MeSH$$aModels, Biological
000058486 650_2 $$2MeSH$$aMolecular Mimicry
000058486 650_2 $$2MeSH$$aUnilamellar Liposomes: chemistry
000058486 650_2 $$2MeSH$$aUnilamellar Liposomes: metabolism
000058486 650_7 $$00$$2NLM Chemicals$$aUnilamellar Liposomes
000058486 650_7 $$01405-69-2$$2NLM Chemicals$$aAvidin
000058486 650_7 $$09004-61-9$$2NLM Chemicals$$aHyaluronic Acid
000058486 650_7 $$2WoSType$$aJ
000058486 7001_ $$0P:(DE-Juel1)VDB57655$$aSengupta, K.$$b1$$uFZJ
000058486 773__ $$0PERI:(DE-600)1477214-0$$a10.1529/biophysj.107.105544$$gVol. 93, p. 3300 - 3313$$p3300 - 3313$$q93<3300 - 3313$$tBiophysical journal$$v93$$x0006-3495$$y2007
000058486 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025668
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000058486 9141_ $$y2007
000058486 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000058486 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0
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