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@ARTICLE{Limozin:58486,
      author       = {Limozin, L. and Sengupta, K.},
      title        = {{M}odulation of {V}esicle {A}dhesion and {S}preading
                      {K}inetics by {H}yaluronan {C}ushions},
      journal      = {Biophysical journal},
      volume       = {93},
      issn         = {0006-3495},
      address      = {New York, NY},
      publisher    = {Rockefeller Univ. Press},
      reportid     = {PreJuSER-58486},
      pages        = {3300 - 3313},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The adhesion of giant unilamellar phospholipid vesicles to
                      planar substrates coated with extracellular matrix mimetic
                      cushions of hyaluronan is studied using quantitative
                      reflection interference contrast microscopy. The absolute
                      height of the vesicle membrane at the vicinity of the
                      substrate is measured by considering, for the first time,
                      the refractive indices of the reflecting media. The
                      thickness of the cushion is varied in the range of
                      approximately 50-100 nm, by designing various coupling
                      strategies. On bare protein-coated substrates, the vesicles
                      spread fast (0.5 s) and form a uniform adhesion disk, with
                      the average membrane height approximately 4 nm. On thick
                      hyaluronan cushions (>80 nm), the membrane height is
                      approximately the same as the thickness of the cushion,
                      implying that the vesicle lies on top of the cushion. On a
                      thin and inhomogeneous hyaluronan cushion, the adhesion is
                      modified but not prevented. The spreading is slow (
                      approximately 20 s) compared to the no-cushion case. The
                      average membrane height is approximately 10 nm and the
                      adhesion disk is studded with blisterlike structures.
                      Observations with fluorescent hyaluronan indicate that the
                      polymer is compressed under, rather than expelled from, the
                      adhesion disk. The adhesion energy density is approximately
                      threefold higher in the no-cushion case (1.2 microJ/m(2)) as
                      compared to the thin-cushion case (0.54 microJ/m(2)). In the
                      thin-cushion case, the presence of short ( approximately 4
                      nm) glyco-polymers on the vesicles results in a hitherto
                      unreported stable partial adhesion state--the membrane
                      height ranges from zero to approximately 250 nm. The minimal
                      model system presented here mimics in vitro the
                      hyaluronan-modulated early stages of cell adhesion, and
                      demonstrates that the presence of a polymer cushion
                      influences both the final equilibrium adhesion-state and the
                      spreading kinetics.},
      keywords     = {Avidin: chemistry / Hyaluronic Acid: chemistry / Hyaluronic
                      Acid: physiology / Kinetics / Microscopy, Interference /
                      Models, Biological / Molecular Mimicry / Unilamellar
                      Liposomes: chemistry / Unilamellar Liposomes: metabolism /
                      Unilamellar Liposomes (NLM Chemicals) / Avidin (NLM
                      Chemicals) / Hyaluronic Acid (NLM Chemicals) / J (WoSType)},
      cin          = {IBN-4},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB802},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK414},
      shelfmark    = {Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17631530},
      pmc          = {pmc:PMC2025668},
      UT           = {WOS:000250199300035},
      doi          = {10.1529/biophysj.107.105544},
      url          = {https://juser.fz-juelich.de/record/58486},
}