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@ARTICLE{Merkel:58487,
author = {Merkel, R. and Kirchgeßner, N. and Cesa, C. M. and
Hoffmann, B.},
title = {{C}ell {F}orce {M}icroscopy on {E}lastic {L}ayers of
{F}inite {T}hickness},
journal = {Biophysical journal},
volume = {93},
issn = {0006-3495},
address = {New York, NY},
publisher = {Rockefeller Univ. Press},
reportid = {PreJuSER-58487},
pages = {3314 - 3323},
year = {2007},
note = {Record converted from VDB: 12.11.2012},
abstract = {Forces applied by cells to substrates can be measured using
soft substrates with embedded displacement markers. Traction
forces are retrieved from microscopic images by determining
the displacements of these markers and fitting the
generating forces. Here we show that using elastic films of
5-10-microm thickness one can improve the spatial resolution
of the technique. To this end we derived explicit equations
for the mechanical response of an elastic layer of finite
thickness to point forces. Moreover, these equations allow
highly accurate force measurements on eukaryotic cells on
films where finite thickness effects are relevant (below
approximately 60 microm).},
keywords = {Animals / Elasticity / Fibroblasts: ultrastructure /
Microscopy, Atomic Force / Myocytes, Cardiac: ultrastructure
/ Rats / Rats, Wistar / Silicon / Silicon (NLM Chemicals) /
J (WoSType)},
cin = {IBN-4},
ddc = {570},
cid = {I:(DE-Juel1)VDB802},
pnm = {Kondensierte Materie},
pid = {G:(DE-Juel1)FUEK414},
shelfmark = {Biophysics},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:17660320},
pmc = {pmc:PMC2025665},
UT = {WOS:000250199300036},
doi = {10.1529/biophysj.107.111328},
url = {https://juser.fz-juelich.de/record/58487},
}