TY  - JOUR
AU  - Wrobel, G.
AU  - Höller, M.
AU  - Ingebrandt, S.
AU  - Dieluweit, S.
AU  - Sommerhage, F.
AU  - Bochem, H. P.
AU  - Offenhäusser, A.
TI  - Transmission electron microscopy study of the cell-sensor interface
JO  - Interface
VL  - 5
SN  - 1742-5689
CY  - London
PB  - The Royal Society
M1  - PreJuSER-58488
SP  - 1094 - 1098
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - An emerging number of micro- and nanoelectronics-based biosensors have been developed for non-invasive recordings of physiological cellular activity. The interface between the biological system and the electronic devices strongly influences the signal transfer between these systems. Little is known about the nanoscopic structure of the cell-sensor interface that is essential for a detailed interpretation of the recordings. Therefore, we analysed the interface between the sensor surface and attached cells using transmission electron microscopy (TEM). The maximum possible resolution of our TEM study, however, was restricted by the quality of the interface preparation. Therefore, we complemented our studies with imaging ellipsometry. We cultured HEK293 cells on substrates, which had been precoated with different types of proteins. We found that contact geometry between attached cell membrane and substrate was dependent on the type of protein coating used. In the presence of polylysine, the average distance of the membrane-substrate interface was in the range of 35-40 nm. However, the cell membrane was highly protruded in the presence of other proteins like fibronectin, laminin or concanavalin-A. The presented method allows the nanoscopic characterization of the cell-sensor interface.
KW  - Cell Adhesion: physiology
KW  - Cell Line
KW  - Cell Membrane: chemistry
KW  - Cell Membrane: ultrastructure
KW  - Epithelial Cells: cytology
KW  - Humans
KW  - Microscopy, Electron, Transmission
KW  - Proteins: metabolism
KW  - Silicon
KW  - Surface Properties
KW  - Proteins (NLM Chemicals)
KW  - Silicon (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17609177
C2  - pmc:PMC2705973
UR  - <Go to ISI:>//WOS:000251628300006
DO  - DOI:10.1098/rsif.2007.1094
UR  - https://juser.fz-juelich.de/record/58488
ER  -