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@ARTICLE{Wrobel:58488,
      author       = {Wrobel, G. and Höller, M. and Ingebrandt, S. and
                      Dieluweit, S. and Sommerhage, F. and Bochem, H. P. and
                      Offenhäusser, A.},
      title        = {{T}ransmission electron microscopy study of the cell-sensor
                      interface},
      journal      = {Interface},
      volume       = {5},
      issn         = {1742-5689},
      address      = {London},
      publisher    = {The Royal Society},
      reportid     = {PreJuSER-58488},
      pages        = {1094 - 1098},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {An emerging number of micro- and nanoelectronics-based
                      biosensors have been developed for non-invasive recordings
                      of physiological cellular activity. The interface between
                      the biological system and the electronic devices strongly
                      influences the signal transfer between these systems. Little
                      is known about the nanoscopic structure of the cell-sensor
                      interface that is essential for a detailed interpretation of
                      the recordings. Therefore, we analysed the interface between
                      the sensor surface and attached cells using transmission
                      electron microscopy (TEM). The maximum possible resolution
                      of our TEM study, however, was restricted by the quality of
                      the interface preparation. Therefore, we complemented our
                      studies with imaging ellipsometry. We cultured HEK293 cells
                      on substrates, which had been precoated with different types
                      of proteins. We found that contact geometry between attached
                      cell membrane and substrate was dependent on the type of
                      protein coating used. In the presence of polylysine, the
                      average distance of the membrane-substrate interface was in
                      the range of 35-40 nm. However, the cell membrane was highly
                      protruded in the presence of other proteins like
                      fibronectin, laminin or concanavalin-A. The presented method
                      allows the nanoscopic characterization of the cell-sensor
                      interface.},
      keywords     = {Cell Adhesion: physiology / Cell Line / Cell Membrane:
                      chemistry / Cell Membrane: ultrastructure / Epithelial
                      Cells: cytology / Humans / Microscopy, Electron,
                      Transmission / Proteins: metabolism / Silicon / Surface
                      Properties / Proteins (NLM Chemicals) / Silicon (NLM
                      Chemicals) / J (WoSType)},
      cin          = {IBN-2 / IBN-4},
      ddc          = {500},
      cid          = {I:(DE-Juel1)IBN-2-20090406 / I:(DE-Juel1)VDB802},
      pnm          = {Kondensierte Materie},
      pid          = {G:(DE-Juel1)FUEK414},
      shelfmark    = {Multidisciplinary Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17609177},
      pmc          = {pmc:PMC2705973},
      UT           = {WOS:000251628300006},
      doi          = {10.1098/rsif.2007.1094},
      url          = {https://juser.fz-juelich.de/record/58488},
}