TY  - JOUR
AU  - Lühring, H.
AU  - Nguyen, V. D.
AU  - Schmidt, L.
AU  - Röse, U. S. R.
TI  - Caterpillar regurgitant induces pore formation in plant membranes
JO  - FEBS letters
VL  - 581
SN  - 0014-5793
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - PreJuSER-58540
SP  - 5361 - 5370
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Formation of channel-like pores in a plant membrane was induced within seconds after application of an aqueous solution containing regurgitant of the insect larvae Spodoptera littoralis. Gated pore currents recorded on the tonoplast of the Charophyte Chara corallina displayed conductances up to several hundred pS. A voltage-dependent gating reaction supports the assumption that pore-forming molecules have amphipathic properties. Regurgitant samples separated into masses smaller or larger than 3kDa were evaluated by patch-clamp and mass spectroscopy. Fractions containing peptides larger than 3kDa constituted pores of large conductances, peptides smaller than 3kDa constituted pores of small conductances. Peptide-free eluates did not constitute conducting pores, indicating that pore-forming components in regurgitant are membrane-spanning oligopeptides.
KW  - Animals
KW  - Characeae
KW  - Electrophysiology
KW  - Mass Spectrometry
KW  - Membranes
KW  - Patch-Clamp Techniques
KW  - Spodoptera
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17967419
UR  - <Go to ISI:>//WOS:000253487900005
DO  - DOI:10.1016/j.febslet.2007.10.035
UR  - https://juser.fz-juelich.de/record/58540
ER  -