000058812 001__ 58812
000058812 005__ 20200402210418.0
000058812 0247_ $$2pmid$$apmid:17981895
000058812 0247_ $$2pmc$$apmc:PMC2242761
000058812 0247_ $$2DOI$$a10.1529/biophysj.107.115766
000058812 0247_ $$2WOS$$aWOS:000253313800037
000058812 037__ $$aPreJuSER-58812
000058812 041__ $$aeng
000058812 082__ $$a570
000058812 084__ $$2WoS$$aBiophysics
000058812 1001_ $$0P:(DE-Juel1)VDB71376$$aKajzar, A.$$b0$$uFZJ
000058812 245__ $$aTowards Physiological Condtions for Cell Analyses: Forces of Heart Muscle Cells Suspended between Elastic Micropillars
000058812 260__ $$aNew York, NY$$bRockefeller Univ. Press$$c2008
000058812 300__ $$a1854 - 1866
000058812 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000058812 3367_ $$2DataCite$$aOutput Types/Journal article
000058812 3367_ $$00$$2EndNote$$aJournal Article
000058812 3367_ $$2BibTeX$$aARTICLE
000058812 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000058812 3367_ $$2DRIVER$$aarticle
000058812 440_0 $$0882$$aBiophysical Journal$$v94$$x0006-3495$$y5
000058812 500__ $$aRecord converted from VDB: 12.11.2012
000058812 520__ $$aAlmost each mammalian cell permanently applies forces to its environment. These forces are essential for many vital processes such as tissue formation or cell movement. In turn, the environmental conditions of cells strongly affect force production. Here we report on the development of an array of elastomeric micropillars as cellular environment. Within these micropillar arrays, we cultivated rat heart muscle cells (cardiac myocytes). For lattice constants between 20 and 30 mum, cells strongly preferred spanning between the elastic micropillars over adhering to the underlying flat substrate. In addition, the architectures of the cytoskeleton and of protein complexes formed for adhesion were strongly dependent on the environment of the cell. On flat parts of the substrates, we observed prominent stress fibers and focal adhesion sites. In contrast, cells suspended between micropillars exhibited well organized myofibers and costameric adhesions at the locations of Z-bands. These observations argue for close-to-nature environmental conditions within micropillar arrays. Resting as well as contraction forces of myocytes resulted in measurable pillar bending. Using an approximate theoretical treatment of elastically founded micropillars, we calculated average cell forces of 140 nN in the relaxed and 400 nN in the contracted state.
000058812 536__ $$0G:(DE-Juel1)FUEK414$$2G:(DE-HGF)$$aKondensierte Materie$$cP54$$x0
000058812 588__ $$aDataset connected to Web of Science, Pubmed
000058812 650_2 $$2MeSH$$aAdherens Junctions: metabolism
000058812 650_2 $$2MeSH$$aAlgorithms
000058812 650_2 $$2MeSH$$aAnimals
000058812 650_2 $$2MeSH$$aCell Adhesion: physiology
000058812 650_2 $$2MeSH$$aCell Membrane: metabolism
000058812 650_2 $$2MeSH$$aCell Movement: physiology
000058812 650_2 $$2MeSH$$aCell Physiological Phenomena
000058812 650_2 $$2MeSH$$aCells, Cultured
000058812 650_2 $$2MeSH$$aCytoskeleton: metabolism
000058812 650_2 $$2MeSH$$aElastomers: chemistry
000058812 650_2 $$2MeSH$$aMicroscopy, Fluorescence
000058812 650_2 $$2MeSH$$aMicroscopy, Immunoelectron
000058812 650_2 $$2MeSH$$aMuscle Proteins: metabolism
000058812 650_2 $$2MeSH$$aMyocytes, Cardiac: cytology
000058812 650_2 $$2MeSH$$aMyocytes, Cardiac: physiology
000058812 650_2 $$2MeSH$$aRats
000058812 650_2 $$2MeSH$$aRats, Wistar
000058812 650_2 $$2MeSH$$aStress, Mechanical
000058812 650_2 $$2MeSH$$aTissue Engineering
000058812 650_7 $$00$$2NLM Chemicals$$aElastomers
000058812 650_7 $$00$$2NLM Chemicals$$aMuscle Proteins
000058812 650_7 $$2WoSType$$aJ
000058812 7001_ $$0P:(DE-Juel1)VDB57503$$aCesa, C. M.$$b1$$uFZJ
000058812 7001_ $$0P:(DE-Juel1)VDB8902$$aKirchgeßner, N.$$b2$$uFZJ
000058812 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b3$$uFZJ
000058812 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b4$$uFZJ
000058812 773__ $$0PERI:(DE-600)1477214-0$$a10.1529/biophysj.107.115766$$gVol. 94, p. 1854 - 1866$$n5$$p1854 - 1866$$q94<1854 - 1866$$tBiophysical journal$$v94$$x0006-3495$$y2008
000058812 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242761
000058812 909CO $$ooai:juser.fz-juelich.de:58812$$pVDB
000058812 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000058812 9141_ $$aNachtrag$$y2008
000058812 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt   bis 2009
000058812 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0
000058812 970__ $$aVDB:(DE-Juel1)92673
000058812 980__ $$aVDB
000058812 980__ $$aConvertedRecord
000058812 980__ $$ajournal
000058812 980__ $$aI:(DE-Juel1)ICS-7-20110106
000058812 980__ $$aUNRESTRICTED
000058812 981__ $$aI:(DE-Juel1)IBI-2-20200312
000058812 981__ $$aI:(DE-Juel1)ICS-7-20110106