TY  - JOUR
AU  - Hartwig, B.
AU  - Borm, B.
AU  - Schneider, H.
AU  - Arin, M. J.
AU  - Kirfel, G.
AU  - Herzog, V.
TI  - Laminin-5-deficient human keratinocytes: Defective adhesion results in a saltatory and inefficient mode of migration
JO  - Experimental cell research
VL  - 313
SN  - 0014-4827
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-59197
SP  - 1575 - 1587
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Laminin-5 is a major adhesion protein of the skin basement membrane and crucially involved in integrin-mediated cell substrate attachment of keratinocytes, which is important for hemidesmosomal anchorage as well as for keratinocyte migration during epidermal wound healing. To investigate its role in keratinocyte migration, we analyzed laminin-5-deficient cells of patients with a lethal variant of junctional epidermolysis bullosa. Normal migrating keratinocytes adopted monopolar morphology with a distinct front lamella and employed a continuous mode of translocation. In contrast, laminin-5-deficient cells assumed a stretched bipolar shape with two lamella regions and migrated in a discontinuous, saltatory manner characterized by significantly decreased directional persistence and reduced migration velocity. The distinct morphology as well as the migratory phenotype apparently resulted from a defect in the formation of cell substrate adhesions that were completely missing in the cell body and less stable in the lamella regions. Accordingly in normal keratinocytes, a bipolar shape and a saltatory migration mode were inducible by blocking laminin-5-mediated substrate adhesion. Our findings clearly point to an essential role of laminin-5 in forming dynamic cell substrate adhesion during migration of epidermal keratinocytes and provide an explanation for the cellular mechanisms that underlie the lethal form of junctional epidermolysis bullosa.
KW  - Cell Adhesion
KW  - Cell Adhesion Molecules: genetics
KW  - Cell Adhesion Molecules: metabolism
KW  - Cell Adhesion Molecules: physiology
KW  - Cell Movement
KW  - Cell Polarity
KW  - Cell Shape
KW  - Cells, Cultured
KW  - Epidermolysis Bullosa, Junctional: metabolism
KW  - Epidermolysis Bullosa, Junctional: pathology
KW  - Extracellular Matrix Proteins: metabolism
KW  - Humans
KW  - Keratinocytes: physiology
KW  - Pseudopodia: physiology
KW  - Cell Adhesion Molecules (NLM Chemicals)
KW  - Extracellular Matrix Proteins (NLM Chemicals)
KW  - kalinin (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17335805
UR  - <Go to ISI:>//WOS:000246128300006
DO  - DOI:10.1016/j.yexcr.2007.02.003
UR  - https://juser.fz-juelich.de/record/59197
ER  -