TY  - JOUR
AU  - Hummel, G. M.
AU  - Schurr, U.
AU  - Baldwin, I. T.
AU  - Walter, A.
TI  - Herbivore-induced jasmonic acid bursts in leaves of Nicotiana attenuata mediate short-term reductions in root growth
JO  - Plant, cell & environment
VL  - 32
SN  - 0140-7791
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-59274
SP  - 134 - 143
PY  - 2009
N1  - We would like to thank Eva Rothe, Jinsong Wu and Norbert Kirchgessner for their assistance. We are also grateful to Jon F. Fobes (AgroFresh Inc) for the generous gift of 1-MCP. We would like to acknowledge stimulating discussions with all members of the Virtual Institute for Biotic Interactions. This work was financially supported by the Helmholtz Association of German Research Centers.
AB  - Root growth in Nicotiana attenuata is transiently reduced after application of oral secretions (OS) of Manduca sexta larvae to wounds in leaves. Feeding of M. sexta or OS elicitation is known to result in jasmonic acid (JA) and ethylene bursts, and activates a suite of defence responses. Because both plant hormones are known to strongly reduce root growth, their activation might account for the observed reduction of root growth following herbivory. To test this hypothesis, we measured primary root growth with digital image sequence processing at high temporal resolution in antisense-lipoxygenase 3 (asLOX3) and inverted repeat-coronatin-insensitive 1 (irCOI1) seedlings which are impaired in JA biosynthesis and perception, respectively, and wild-type (WT) seedlings. Higher root growth rates in irCOI1 compared with WT were observed after OS elicitation. The dynamics of wound-induced root growth reduction coincide with the dynamics of root growth reduction induced by external application of methyl JA. In an experiment with 1-methylcyclopropen (1-MCP), a potent ethylene receptor blocker, no wounding-specific difference between growth of 1-MCP-treated plants and non-treated plants was observed, suggesting that wound-induced endogenous JA and not ethylene mediates the wounding-specific reduction in root growth. Yet, inhibiting the ethylene response by applying 1-MCP led to markedly increased root growth compared with that of control plants, indicating that ethylene normally suppresses plant growth in N. attenuata seedlings.
KW  - Animals
KW  - Cyclopentanes: metabolism
KW  - Cyclopropanes: pharmacology
KW  - Ethylenes: metabolism
KW  - Gene Expression Regulation, Plant
KW  - Manduca: physiology
KW  - Oxylipins: metabolism
KW  - Plant Growth Regulators: metabolism
KW  - Plant Leaves: genetics
KW  - Plant Leaves: growth & development
KW  - Plant Leaves: metabolism
KW  - Plant Roots: drug effects
KW  - Plant Roots: genetics
KW  - Plant Roots: growth & development
KW  - Plant Roots: metabolism
KW  - Tobacco: drug effects
KW  - Tobacco: genetics
KW  - Tobacco: growth & development
KW  - Tobacco: metabolism
KW  - Cyclopentanes (NLM Chemicals)
KW  - Cyclopropanes (NLM Chemicals)
KW  - Ethylenes (NLM Chemicals)
KW  - Oxylipins (NLM Chemicals)
KW  - Plant Growth Regulators (NLM Chemicals)
KW  - 1-methylcyclopropene (NLM Chemicals)
KW  - jasmonic acid (NLM Chemicals)
KW  - ethylene (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19054344
UR  - <Go to ISI:>//WOS:000262349100004
DO  - DOI:10.1111/j.1365-3040.2008.01905.x
UR  - https://juser.fz-juelich.de/record/59274
ER  -