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000059358 0247_ $$2pmid$$apmid:17548357
000059358 0247_ $$2DOI$$a10.1074/jbc.M700849200
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000059358 084__ $$2WoS$$aBiochemistry & Molecular Biology
000059358 1001_ $$0P:(DE-Juel1)VDB72038$$aImmeln, D.$$b0$$uFZJ
000059358 245__ $$aBlue Light Induces Radical Formation and Autophosphorylation in the Light-sensitive Domain of Chlamydomonas Cryptochrome
000059358 260__ $$aBethesda, Md.$$bSoc.$$c2007
000059358 300__ $$a21720 - 21728
000059358 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000059358 440_0 $$03091$$aJournal of Biological Chemistry$$v282$$x0021-9258$$y30
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000059358 520__ $$aCryptochromes are sensory blue light receptors mediating various responses in plants and animals. Studies on the mechanism of plant cryptochromes have been focused on the flowering plant Arabidopsis. In the genome of the unicellular green alga Chlamydomonas reinhardtii, a single plant cryptochrome, Chlamydomonas photolyase homologue 1 (CPH1), has been identified. The N-terminal 500 amino acids comprise the light-sensitive domain of CPH1 linked to a C-terminal extension of similar size. We have expressed the light-sensitive domain heterologously in Escherichia coli in high yield and purity. The 59-kDa protein bears exclusively flavin adenine dinucleotide in its oxidized state. Illumination with blue light induces formation of a neutral flavin radical with absorption maxima at 540 and 580 nm. The reaction proceeds aerobically even in the absence of an exogenous electron donor, which suggests that it reflects a physiological response. The process is completely reversible in the dark and exhibits a decay time constant of 200 s in the presence of oxygen. Binding of ATP strongly stabilizes the radical state after illumination and impedes the dark recovery. Thus, ATP binding has functional significance for plant cryptochromes and does not merely result from structural homology to DNA photolyase. The light-sensitive domain responds to illumination by an increase in phosphorylation. The autophosphorylation takes place although the protein is lacking its native C-terminal extension. This finding indicates that the extension is dispensable for autophosphorylation, despite the role it has been assigned in mediating signal transduction in Arabidopsis.
000059358 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000059358 588__ $$aDataset connected to Web of Science, Pubmed
000059358 650_2 $$2MeSH$$aAnimals
000059358 650_2 $$2MeSH$$aChlamydomonas: physiology
000059358 650_2 $$2MeSH$$aChlamydomonas: radiation effects
000059358 650_2 $$2MeSH$$aCryptochromes
000059358 650_2 $$2MeSH$$aDNA, Protozoan: genetics
000059358 650_2 $$2MeSH$$aDNA, Protozoan: isolation & purification
000059358 650_2 $$2MeSH$$aEscherichia coli: genetics
000059358 650_2 $$2MeSH$$aFlavoproteins: genetics
000059358 650_2 $$2MeSH$$aFlavoproteins: metabolism
000059358 650_2 $$2MeSH$$aFlavoproteins: radiation effects
000059358 650_2 $$2MeSH$$aFree Radicals
000059358 650_2 $$2MeSH$$aKinetics
000059358 650_2 $$2MeSH$$aLight
000059358 650_2 $$2MeSH$$aLight Signal Transduction
000059358 650_2 $$2MeSH$$aPhosphorylation
000059358 650_2 $$2MeSH$$aProtozoan Proteins: metabolism
000059358 650_2 $$2MeSH$$aProtozoan Proteins: radiation effects
000059358 650_2 $$2MeSH$$aRecombinant Proteins: metabolism
000059358 650_2 $$2MeSH$$aRecombinant Proteins: radiation effects
000059358 650_7 $$00$$2NLM Chemicals$$aCryptochromes
000059358 650_7 $$00$$2NLM Chemicals$$aDNA, Protozoan
000059358 650_7 $$00$$2NLM Chemicals$$aFlavoproteins
000059358 650_7 $$00$$2NLM Chemicals$$aFree Radicals
000059358 650_7 $$00$$2NLM Chemicals$$aProtozoan Proteins
000059358 650_7 $$00$$2NLM Chemicals$$aRecombinant Proteins
000059358 650_7 $$2WoSType$$aJ
000059358 7001_ $$0P:(DE-Juel1)VDB1421$$aSchlesinger, R.$$b1$$uFZJ
000059358 7001_ $$0P:(DE-HGF)0$$aHeberle, J.$$b2
000059358 7001_ $$0P:(DE-Juel1)VDB16784$$aKottke, T.$$b3$$uFZJ
000059358 773__ $$0PERI:(DE-600)1474604-9$$a10.1074/jbc.M700849200$$gVol. 282, p. 21720 - 21728$$p21720 - 21728$$q282<21720 - 21728$$tThe @journal of biological chemistry$$v282$$x0021-9258$$y2007
000059358 8567_ $$uhttp://dx.doi.org/10.1074/jbc.M700849200
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000059358 9141_ $$y2007
000059358 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000059358 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x1
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