000059381 001__ 59381
000059381 005__ 20200423204545.0
000059381 0247_ $$2pmid$$apmid:17552908
000059381 0247_ $$2DOI$$a10.1515/BC.2007.075
000059381 0247_ $$2WOS$$aWOS:000247141500007
000059381 0247_ $$2Handle$$a2128/18399
000059381 0247_ $$2altmetric$$aaltmetric:21818079
000059381 037__ $$aPreJuSER-59381
000059381 041__ $$aeng
000059381 082__ $$a540
000059381 084__ $$2WoS$$aBiochemistry & Molecular Biology
000059381 1001_ $$0P:(DE-Juel1)VDB8627$$aStangler, T.$$b0$$uFZJ
000059381 245__ $$aCompetitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide
000059381 260__ $$aBerlin [u.a.]$$bde Gruyter$$c2007
000059381 300__ $$a611 - 615
000059381 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000059381 3367_ $$2DataCite$$aOutput Types/Journal article
000059381 3367_ $$00$$2EndNote$$aJournal Article
000059381 3367_ $$2BibTeX$$aARTICLE
000059381 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000059381 3367_ $$2DRIVER$$aarticle
000059381 440_0 $$09042$$aBiological Chemistry$$v388$$x1431-6730$$y6
000059381 500__ $$aRecord converted from VDB: 12.11.2012
000059381 520__ $$aWe studied the interaction of the artificial 12-aa proline-rich peptide PD1 with the SH3 domain of the hematopoietic cell kinase Hck and the peptide's potency in competitively displacing HIV-1 Nef from the Hck SH3 domain. PD1 was obtained from a phage display screen and exhibits exceptional affinity for the Hck SH3 domain (K(d)=0.23 microM). Competition experiments using NMR spectroscopy demonstrate that the peptide even displaces Nef from Hck SH3 and allow for estimation of the Nef-Hck SH3 dissociation constant (K(d)=0.44 microM), the strongest SH3 ligand interaction known so far. Consequences of this study for novel antiviral concepts are discussed.
000059381 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000059381 588__ $$aDataset connected to Web of Science, Pubmed
000059381 650_2 $$2MeSH$$aBinding, Competitive
000059381 650_2 $$2MeSH$$aGene Products, nef: metabolism
000059381 650_2 $$2MeSH$$aHumans
000059381 650_2 $$2MeSH$$aPeptides: metabolism
000059381 650_2 $$2MeSH$$aProtein Binding
000059381 650_2 $$2MeSH$$aProto-Oncogene Proteins c-hck: metabolism
000059381 650_2 $$2MeSH$$anef Gene Products, Human Immunodeficiency Virus
000059381 650_2 $$2MeSH$$asrc Homology Domains
000059381 650_7 $$00$$2NLM Chemicals$$aGene Products, nef
000059381 650_7 $$00$$2NLM Chemicals$$aPeptides
000059381 650_7 $$00$$2NLM Chemicals$$anef Gene Products, Human Immunodeficiency Virus
000059381 650_7 $$0EC 2.7.10.2$$2NLM Chemicals$$aHCK protein, human
000059381 650_7 $$0EC 2.7.10.2$$2NLM Chemicals$$aProto-Oncogene Proteins c-hck
000059381 650_7 $$2WoSType$$aJ
000059381 65320 $$2Author$$acompetition
000059381 65320 $$2Author$$aHck
000059381 65320 $$2Author$$aHIV
000059381 65320 $$2Author$$aNef
000059381 65320 $$2Author$$aNMR
000059381 65320 $$2Author$$aprotein-peptide interaction
000059381 65320 $$2Author$$aSH3
000059381 7001_ $$0P:(DE-Juel1)162212$$aTran, T.$$b1$$uFZJ
000059381 7001_ $$0P:(DE-Juel1)VDB630$$aHoffmann, S.$$b2$$uFZJ
000059381 7001_ $$0P:(DE-Juel1)VDB2063$$aSchmidt, H.$$b3$$uFZJ
000059381 7001_ $$0P:(DE-Juel1)VDB20019$$aJonas, E.$$b4$$uFZJ
000059381 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b5$$uFZJ
000059381 773__ $$0PERI:(DE-600)1466062-3$$a10.1515/BC.2007.075$$gVol. 388, p. 611 - 615$$p611 - 615$$q388<611 - 615$$tBiological chemistry$$v388$$x1431-6730$$y2007
000059381 8567_ $$uhttp://dx.doi.org/10.1515/BC.2007.075
000059381 8564_ $$uhttps://juser.fz-juelich.de/record/59381/files/%5BBiological%20Chemistry%5D%20Competitive%20displacement%20of%20full-length%20HIV-1%20Nef%20from%20the%20Hck%20SH3%20domain%20by%20a%20high-affinity%20artificial%20peptide.pdf$$yOpenAccess
000059381 8564_ $$uhttps://juser.fz-juelich.de/record/59381/files/%5BBiological%20Chemistry%5D%20Competitive%20displacement%20of%20full-length%20HIV-1%20Nef%20from%20the%20Hck%20SH3%20domain%20by%20a%20high-affinity%20artificial%20peptide.gif?subformat=icon$$xicon$$yOpenAccess
000059381 8564_ $$uhttps://juser.fz-juelich.de/record/59381/files/%5BBiological%20Chemistry%5D%20Competitive%20displacement%20of%20full-length%20HIV-1%20Nef%20from%20the%20Hck%20SH3%20domain%20by%20a%20high-affinity%20artificial%20peptide.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000059381 8564_ $$uhttps://juser.fz-juelich.de/record/59381/files/%5BBiological%20Chemistry%5D%20Competitive%20displacement%20of%20full-length%20HIV-1%20Nef%20from%20the%20Hck%20SH3%20domain%20by%20a%20high-affinity%20artificial%20peptide.jpg?subformat=icon-700$$xicon-700$$yOpenAccess
000059381 909CO $$ooai:juser.fz-juelich.de:59381$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000059381 9131_ $$0G:(DE-Juel1)FUEK409$$bGesundheit$$kP33$$lFunktion und Dysfunktion des Nervensystems$$vFunktion und Dysfunktion des Nervensystems$$x0
000059381 9141_ $$y2007
000059381 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000059381 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000059381 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x1
000059381 9201_ $$0I:(DE-Juel1)VDB1045$$gJARA$$kJARA-SIM$$lJülich-Aachen Research Alliance - Simulation Sciences$$x2
000059381 970__ $$aVDB:(DE-Juel1)93427
000059381 9801_ $$aFullTexts
000059381 980__ $$aVDB
000059381 980__ $$aConvertedRecord
000059381 980__ $$ajournal
000059381 980__ $$aI:(DE-Juel1)ISB-2-20090406
000059381 980__ $$aI:(DE-Juel1)VDB1045
000059381 980__ $$aUNRESTRICTED
000059381 980__ $$aI:(DE-Juel1)ICS-6-20110106
000059381 981__ $$aI:(DE-Juel1)IBI-7-20200312
000059381 981__ $$aI:(DE-Juel1)ISB-2-20090406
000059381 981__ $$aI:(DE-Juel1)VDB1045
000059381 981__ $$aI:(DE-Juel1)ICS-6-20110106