Hauptseite > Publikationsdatenbank > Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide > print

001     59381
005     20200423204545.0
024 7 _ |a pmid:17552908
|2 pmid
024 7 _ |a 10.1515/BC.2007.075
|2 DOI
024 7 _ |a WOS:000247141500007
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024 7 _ |a 2128/18399
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037 _ _ |a PreJuSER-59381
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
100 1 _ |a Stangler, T.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB8627
245 _ _ |a Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide
260 _ _ |a Berlin [u.a.]
|b de Gruyter
|c 2007
300 _ _ |a 611 - 615
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a article
|2 DRIVER
440 _ 0 |a Biological Chemistry
|x 1431-6730
|0 9042
|y 6
|v 388
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a We studied the interaction of the artificial 12-aa proline-rich peptide PD1 with the SH3 domain of the hematopoietic cell kinase Hck and the peptide's potency in competitively displacing HIV-1 Nef from the Hck SH3 domain. PD1 was obtained from a phage display screen and exhibits exceptional affinity for the Hck SH3 domain (K(d)=0.23 microM). Competition experiments using NMR spectroscopy demonstrate that the peptide even displaces Nef from Hck SH3 and allow for estimation of the Nef-Hck SH3 dissociation constant (K(d)=0.44 microM), the strongest SH3 ligand interaction known so far. Consequences of this study for novel antiviral concepts are discussed.
536 _ _ |a Funktion und Dysfunktion des Nervensystems
|c P33
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK409
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Binding, Competitive
650 _ 2 |2 MeSH
|a Gene Products, nef: metabolism
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Peptides: metabolism
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a Proto-Oncogene Proteins c-hck: metabolism
650 _ 2 |2 MeSH
|a nef Gene Products, Human Immunodeficiency Virus
650 _ 2 |2 MeSH
|a src Homology Domains
650 _ 7 |0 0
|2 NLM Chemicals
|a Gene Products, nef
650 _ 7 |0 0
|2 NLM Chemicals
|a Peptides
650 _ 7 |0 0
|2 NLM Chemicals
|a nef Gene Products, Human Immunodeficiency Virus
650 _ 7 |0 EC 2.7.10.2
|2 NLM Chemicals
|a HCK protein, human
650 _ 7 |0 EC 2.7.10.2
|2 NLM Chemicals
|a Proto-Oncogene Proteins c-hck
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a competition
653 2 0 |2 Author
|a Hck
653 2 0 |2 Author
|a HIV
653 2 0 |2 Author
|a Nef
653 2 0 |2 Author
|a NMR
653 2 0 |2 Author
|a protein-peptide interaction
653 2 0 |2 Author
|a SH3
700 1 _ |a Tran, T.
|b 1
|u FZJ
|0 P:(DE-Juel1)162212
700 1 _ |a Hoffmann, S.
|b 2
|u FZJ
|0 P:(DE-Juel1)VDB630
700 1 _ |a Schmidt, H.
|b 3
|u FZJ
|0 P:(DE-Juel1)VDB2063
700 1 _ |a Jonas, E.
|b 4
|u FZJ
|0 P:(DE-Juel1)VDB20019
700 1 _ |a Willbold, D.
|b 5
|u FZJ
|0 P:(DE-Juel1)132029
773 _ _ |a 10.1515/BC.2007.075
|g Vol. 388, p. 611 - 615
|p 611 - 615
|q 388<611 - 615
|0 PERI:(DE-600)1466062-3
|t Biological chemistry
|v 388
|y 2007
|x 1431-6730
856 7 _ |u http://dx.doi.org/10.1515/BC.2007.075
856 4 _ |u https://juser.fz-juelich.de/record/59381/files/%5BBiological%20Chemistry%5D%20Competitive%20displacement%20of%20full-length%20HIV-1%20Nef%20from%20the%20Hck%20SH3%20domain%20by%20a%20high-affinity%20artificial%20peptide.pdf
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