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000060108 0247_ $$2DOI$$a10.1016/j.vetmic.2007.04.001
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000060108 041__ $$aeng
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000060108 084__ $$2WoS$$aMicrobiology
000060108 084__ $$2WoS$$aVeterinary Sciences
000060108 1001_ $$0P:(DE-Juel1)VDB65870$$aBirkmann, E.$$b0$$uFZJ
000060108 245__ $$aCounting of single prion particles bound to a capture-antibody surface (surface-FIDA)
000060108 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2007
000060108 300__ $$a294 - 304
000060108 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000060108 440_0 $$018203$$aVeterinary Microbiology$$v123$$x0378-1135
000060108 500__ $$aRecord converted from VDB: 12.11.2012
000060108 520__ $$aHitherto accredited prion tests use the PK resistance of PrP(Sc), the pathogenic isoform of the prion protein, as a marker for the disease. Because of variations in the amount of disease-related aggregated PrP, which is not PK-resistant, these prion tests offer only limited sensitivity. Therefore, a prion detection method that does not rely on PK digestion would allow for the detection of both PK-resistant as well as PK-sensitive PrP(Sc). Furthermore, single particle counting is more sensitive than methods measuring an integrated signal. Our new test system is based on dual-colour fluorescence correlation spectroscopy (FCS). This method quantifies the number of protein aggregates that have been simultaneously labelled with two different antibodies using dual-colour fluorescence intensity distribution analysis (2D-FIDA). This only counts PrP aggregates, and not PrP monomers. To increase the sensitivity, PrP(Sc) was concentrated in a two-dimensional space by immobilizing it so that the antibodies could be captured on the surface of the slide (surface-FIDA). When the surface was systematically scanned, even single prion particles were detected. Using this new technique, the sensitivity to identify samples from scrapie-infected hamster as well as BSE-infected cattle can be dramatically increased in comparison with identification using FIDA in solution.
000060108 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000060108 588__ $$aDataset connected to Web of Science, Pubmed
000060108 650_2 $$2MeSH$$aAnimals
000060108 650_2 $$2MeSH$$aBlotting, Western: veterinary
000060108 650_2 $$2MeSH$$aCattle
000060108 650_2 $$2MeSH$$aCricetinae
000060108 650_2 $$2MeSH$$aElectrophoresis, Polyacrylamide Gel: veterinary
000060108 650_2 $$2MeSH$$aEncephalopathy, Bovine Spongiform: diagnosis
000060108 650_2 $$2MeSH$$aEndopeptidase K: chemistry
000060108 650_2 $$2MeSH$$aEndopeptidase K: metabolism
000060108 650_2 $$2MeSH$$aPrPSc Proteins: cerebrospinal fluid
000060108 650_2 $$2MeSH$$aPrPSc Proteins: isolation & purification
000060108 650_2 $$2MeSH$$aPrion Diseases: diagnosis
000060108 650_2 $$2MeSH$$aPrion Diseases: veterinary
000060108 650_2 $$2MeSH$$aPrions: isolation & purification
000060108 650_2 $$2MeSH$$aScrapie: diagnosis
000060108 650_2 $$2MeSH$$aSensitivity and Specificity
000060108 650_2 $$2MeSH$$aSpectrometry, Fluorescence: methods
000060108 650_2 $$2MeSH$$aSpectrometry, Fluorescence: veterinary
000060108 650_7 $$00$$2NLM Chemicals$$aPrPSc Proteins
000060108 650_7 $$00$$2NLM Chemicals$$aPrions
000060108 650_7 $$0EC 3.4.21.64$$2NLM Chemicals$$aEndopeptidase K
000060108 650_7 $$2WoSType$$aJ
000060108 65320 $$2Author$$aprion
000060108 65320 $$2Author$$aprotemase K-free diagnosis
000060108 65320 $$2Author$$aTSE diagnosis
000060108 65320 $$2Author$$asingle. particle detection
000060108 65320 $$2Author$$aSurface-FIDA
000060108 65320 $$2Author$$aBSE
000060108 65320 $$2Author$$ascrapie
000060108 65320 $$2Author$$aCSF
000060108 7001_ $$0P:(DE-Juel1)VDB65871$$aHenke, F.$$b1$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)VDB72719$$aWeinmann, N$$b2$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)VDB72720$$aDumpitak, C.$$b3$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)VDB72721$$aGroshup, M.$$b4$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)VDB65462$$aFunke, A.$$b5$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b6$$uFZJ
000060108 7001_ $$0P:(DE-Juel1)VDB2263$$aRiesner, D.$$b7$$uFZJ
000060108 773__ $$0PERI:(DE-600)1498996-7$$a10.1016/j.vetmic.2007.04.001$$gVol. 123, p. 294 - 304$$p294 - 304$$q123<294 - 304$$tVeterinary microbiology$$v123$$x0378-1135$$y2007
000060108 8567_ $$uhttp://dx.doi.org/10.1016/j.vetmic.2007.04.001
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