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| Hauptseite > Publikationsdatenbank > Counting of single prion particles bound to a capture-antibody surface (surface-FIDA) > print |
| Hauptseite > Publikationsdatenbank > Counting of single prion particles bound to a capture-antibody surface (surface-FIDA) > print |
| 001 | 60108 | ||
| 005 | 20200402210439.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:17499942 |
| 024 | 7 | _ | |2 DOI |a 10.1016/j.vetmic.2007.04.001 |
| 024 | 7 | _ | |2 WOS |a WOS:000249306400003 |
| 037 | _ | _ | |a PreJuSER-60108 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 630 |
| 084 | _ | _ | |2 WoS |a Microbiology |
| 084 | _ | _ | |2 WoS |a Veterinary Sciences |
| 100 | 1 | _ | |a Birkmann, E. |b 0 |u FZJ |0 P:(DE-Juel1)VDB65870 |
| 245 | _ | _ | |a Counting of single prion particles bound to a capture-antibody surface (surface-FIDA) |
| 260 | _ | _ | |a Amsterdam [u.a.] |b Elsevier Science |c 2007 |
| 300 | _ | _ | |a 294 - 304 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Veterinary Microbiology |x 0378-1135 |0 18203 |v 123 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Hitherto accredited prion tests use the PK resistance of PrP(Sc), the pathogenic isoform of the prion protein, as a marker for the disease. Because of variations in the amount of disease-related aggregated PrP, which is not PK-resistant, these prion tests offer only limited sensitivity. Therefore, a prion detection method that does not rely on PK digestion would allow for the detection of both PK-resistant as well as PK-sensitive PrP(Sc). Furthermore, single particle counting is more sensitive than methods measuring an integrated signal. Our new test system is based on dual-colour fluorescence correlation spectroscopy (FCS). This method quantifies the number of protein aggregates that have been simultaneously labelled with two different antibodies using dual-colour fluorescence intensity distribution analysis (2D-FIDA). This only counts PrP aggregates, and not PrP monomers. To increase the sensitivity, PrP(Sc) was concentrated in a two-dimensional space by immobilizing it so that the antibodies could be captured on the surface of the slide (surface-FIDA). When the surface was systematically scanned, even single prion particles were detected. Using this new technique, the sensitivity to identify samples from scrapie-infected hamster as well as BSE-infected cattle can be dramatically increased in comparison with identification using FIDA in solution. |
| 536 | _ | _ | |a Funktion und Dysfunktion des Nervensystems |c P33 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK409 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Animals |
| 650 | _ | 2 | |2 MeSH |a Blotting, Western: veterinary |
| 650 | _ | 2 | |2 MeSH |a Cattle |
| 650 | _ | 2 | |2 MeSH |a Cricetinae |
| 650 | _ | 2 | |2 MeSH |a Electrophoresis, Polyacrylamide Gel: veterinary |
| 650 | _ | 2 | |2 MeSH |a Encephalopathy, Bovine Spongiform: diagnosis |
| 650 | _ | 2 | |2 MeSH |a Endopeptidase K: chemistry |
| 650 | _ | 2 | |2 MeSH |a Endopeptidase K: metabolism |
| 650 | _ | 2 | |2 MeSH |a PrPSc Proteins: cerebrospinal fluid |
| 650 | _ | 2 | |2 MeSH |a PrPSc Proteins: isolation & purification |
| 650 | _ | 2 | |2 MeSH |a Prion Diseases: diagnosis |
| 650 | _ | 2 | |2 MeSH |a Prion Diseases: veterinary |
| 650 | _ | 2 | |2 MeSH |a Prions: isolation & purification |
| 650 | _ | 2 | |2 MeSH |a Scrapie: diagnosis |
| 650 | _ | 2 | |2 MeSH |a Sensitivity and Specificity |
| 650 | _ | 2 | |2 MeSH |a Spectrometry, Fluorescence: methods |
| 650 | _ | 2 | |2 MeSH |a Spectrometry, Fluorescence: veterinary |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a PrPSc Proteins |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Prions |
| 650 | _ | 7 | |0 EC 3.4.21.64 |2 NLM Chemicals |a Endopeptidase K |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a prion |
| 653 | 2 | 0 | |2 Author |a protemase K-free diagnosis |
| 653 | 2 | 0 | |2 Author |a TSE diagnosis |
| 653 | 2 | 0 | |2 Author |a single. particle detection |
| 653 | 2 | 0 | |2 Author |a Surface-FIDA |
| 653 | 2 | 0 | |2 Author |a BSE |
| 653 | 2 | 0 | |2 Author |a scrapie |
| 653 | 2 | 0 | |2 Author |a CSF |
| 700 | 1 | _ | |a Henke, F. |b 1 |u FZJ |0 P:(DE-Juel1)VDB65871 |
| 700 | 1 | _ | |a Weinmann, N |b 2 |u FZJ |0 P:(DE-Juel1)VDB72719 |
| 700 | 1 | _ | |a Dumpitak, C. |b 3 |u FZJ |0 P:(DE-Juel1)VDB72720 |
| 700 | 1 | _ | |a Groshup, M. |b 4 |u FZJ |0 P:(DE-Juel1)VDB72721 |
| 700 | 1 | _ | |a Funke, A. |b 5 |u FZJ |0 P:(DE-Juel1)VDB65462 |
| 700 | 1 | _ | |a Willbold, D. |b 6 |u FZJ |0 P:(DE-Juel1)132029 |
| 700 | 1 | _ | |a Riesner, D. |b 7 |u FZJ |0 P:(DE-Juel1)VDB2263 |
| 773 | _ | _ | |a 10.1016/j.vetmic.2007.04.001 |g Vol. 123, p. 294 - 304 |p 294 - 304 |q 123<294 - 304 |0 PERI:(DE-600)1498996-7 |t Veterinary microbiology |v 123 |y 2007 |x 0378-1135 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1016/j.vetmic.2007.04.001 |
| 909 | C | O | |o oai:juser.fz-juelich.de:60108 |p VDB |
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| 914 | 1 | _ | |y 2007 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k INB-2 |l Molekulare Biophysik |d 31.12.2008 |g INB |0 I:(DE-Juel1)VDB805 |x 0 |
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| 980 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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| 981 | _ | _ | |a I:(DE-Juel1)ISB-2-20090406 |
| 981 | _ | _ | |a I:(DE-Juel1)ICS-6-20110106 |
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