TY  - JOUR
AU  - Funke, S. A.
AU  - Birkmann, E.
AU  - Henke, F.
AU  - Görtz, P.
AU  - Lange-Asschenfeldt, C.
AU  - Riesner, D.
AU  - Willbold, D.
TI  - Single particle detection of amyloid-ß aggregates associated with Alzheimer's disease
JO  - Biochemical and biophysical research communications
VL  - 364
SN  - 0006-291X
CY  - Orlando, Fla.
PB  - Academic Press
M1  - PreJuSER-60109
SP  - 902 - 907
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the most common cause of dementia. Today, AD can be diagnosed with certainty only post-mortem, by histopathologic staining of Abeta plaques and neurofibrillary tangles in brain tissue sections. We have developed an ultra-sensitive assay potentially suitable for early and non-invasive diagnosis of AD. This highly specific and sensitive assay uses fluorescence correlation spectroscopy (FCS) and is sensitive enough to detect even single aggregates in body fluids of AD patients. First results show a clear distinction between AD diseased people and non-demented controls by analysing cerebrospinal fluids (CSF) by confocal scanning of surface captured Abeta aggregates and subsequent two-dimensional fluorescence intensity distribution analysis.
KW  - Alzheimer Disease: cerebrospinal fluid
KW  - Alzheimer Disease: diagnosis
KW  - Amyloid beta-Peptides: analysis
KW  - Amyloid beta-Peptides: ultrastructure
KW  - Cerebrospinal Fluid: cytology
KW  - Cerebrospinal Fluid: metabolism
KW  - Humans
KW  - Image Enhancement: methods
KW  - Microscopy, Confocal: methods
KW  - Microscopy, Fluorescence: methods
KW  - Particle Size
KW  - Amyloid beta-Peptides (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17963690
UR  - <Go to ISI:>//WOS:000251358500031
DO  - DOI:10.1016/j.bbrc.2007.10.085
UR  - https://juser.fz-juelich.de/record/60109
ER  -