TY  - JOUR
AU  - Hänel, K.
AU  - Willbold, D.
TI  - SARS-CoV accessory protein 7a directly interacts with human LFA-1
JO  - Biological chemistry
VL  - 388
SN  - 1431-6730
CY  - Berlin [u.a.]
PB  - de Gruyter
M1  - PreJuSER-60124
SP  - 1325 - 1332
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - The SARS-CoV accessory protein 7a is a type I membrane protein with an extracellular domain of 81 amino acid residues. It is described to be expressed during infection and to be a component of the virus particle surface. In this study, we demonstrate that protein 7a binds directly and specifically to human lymphocyte function-associated antigen 1 (LFA-1) on the cell surface of Jurkat cells. The binding is increased upon artificial cell activation with phorbol ester. These observations are confirmed by direct in vitro binding of recombinant protein 7a to the wild type and mutant K287C/K294C I domain showing that the I domain is the 7a binding site in the alpha(L) chain of LFA-1. Consequences of the LFA-1 interaction with 7a are discussed. In particular, our data suggest LFA-1 to be an attachment factor or the receptor for SARS-CoV on human leukocytes.
KW  - Blotting, Western
KW  - Carcinogens: pharmacology
KW  - Cloning, Molecular
KW  - Enzyme-Linked Immunosorbent Assay
KW  - Humans
KW  - Intercellular Adhesion Molecule-1: metabolism
KW  - Jurkat Cells
KW  - Lymphocyte Function-Associated Antigen-1: chemistry
KW  - Mutation
KW  - Phorbol 12,13-Dibutyrate: pharmacology
KW  - Protein Binding
KW  - Protein Conformation
KW  - Receptors, Cell Surface: metabolism
KW  - Recombinant Proteins: chemistry
KW  - Viral Matrix Proteins: chemistry
KW  - Viral Matrix Proteins: genetics
KW  - Viral Proteins: chemistry
KW  - Viral Proteins: genetics
KW  - Carcinogens (NLM Chemicals)
KW  - Lymphocyte Function-Associated Antigen-1 (NLM Chemicals)
KW  - Receptors, Cell Surface (NLM Chemicals)
KW  - Recombinant Proteins (NLM Chemicals)
KW  - Viral Matrix Proteins (NLM Chemicals)
KW  - Viral Proteins (NLM Chemicals)
KW  - sars7a protein, SARS virus (NLM Chemicals)
KW  - Intercellular Adhesion Molecule-1 (NLM Chemicals)
KW  - Phorbol 12,13-Dibutyrate (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18020948
UR  - <Go to ISI:>//WOS:000251393700006
DO  - DOI:10.1515/BC.2007.157
UR  - https://juser.fz-juelich.de/record/60124
ER  -