Home > Publications database > SARS-CoV accessory protein 7a directly interacts with human LFA-1 > print

001     60124
005     20200423204551.0
024 7 _ |a pmid:18020948
|2 pmid
024 7 _ |a 10.1515/BC.2007.157
|2 DOI
024 7 _ |a WOS:000251393700006
|2 WOS
024 7 _ |a 2128/18398
|2 Handle
024 7 _ |a altmetric:21818210
|2 altmetric
037 _ _ |a PreJuSER-60124
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
100 1 _ |a Hänel, K.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB21605
245 _ _ |a SARS-CoV accessory protein 7a directly interacts with human LFA-1
260 _ _ |a Berlin [u.a.]
|b de Gruyter
|c 2007
300 _ _ |a 1325 - 1332
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Biological Chemistry
|x 1431-6730
|0 9042
|v 388
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a The SARS-CoV accessory protein 7a is a type I membrane protein with an extracellular domain of 81 amino acid residues. It is described to be expressed during infection and to be a component of the virus particle surface. In this study, we demonstrate that protein 7a binds directly and specifically to human lymphocyte function-associated antigen 1 (LFA-1) on the cell surface of Jurkat cells. The binding is increased upon artificial cell activation with phorbol ester. These observations are confirmed by direct in vitro binding of recombinant protein 7a to the wild type and mutant K287C/K294C I domain showing that the I domain is the 7a binding site in the alpha(L) chain of LFA-1. Consequences of the LFA-1 interaction with 7a are discussed. In particular, our data suggest LFA-1 to be an attachment factor or the receptor for SARS-CoV on human leukocytes.
536 _ _ |a Funktion und Dysfunktion des Nervensystems
|c P33
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK409
|x 0
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Blotting, Western
650 _ 2 |2 MeSH
|a Carcinogens: pharmacology
650 _ 2 |2 MeSH
|a Cloning, Molecular
650 _ 2 |2 MeSH
|a Enzyme-Linked Immunosorbent Assay
650 _ 2 |2 MeSH
|a Humans
650 _ 2 |2 MeSH
|a Intercellular Adhesion Molecule-1: metabolism
650 _ 2 |2 MeSH
|a Jurkat Cells
650 _ 2 |2 MeSH
|a Lymphocyte Function-Associated Antigen-1: chemistry
650 _ 2 |2 MeSH
|a Mutation
650 _ 2 |2 MeSH
|a Phorbol 12,13-Dibutyrate: pharmacology
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a Protein Conformation
650 _ 2 |2 MeSH
|a Receptors, Cell Surface: metabolism
650 _ 2 |2 MeSH
|a Recombinant Proteins: chemistry
650 _ 2 |2 MeSH
|a Viral Matrix Proteins: chemistry
650 _ 2 |2 MeSH
|a Viral Matrix Proteins: genetics
650 _ 2 |2 MeSH
|a Viral Proteins: chemistry
650 _ 2 |2 MeSH
|a Viral Proteins: genetics
650 _ 7 |0 0
|2 NLM Chemicals
|a Carcinogens
650 _ 7 |0 0
|2 NLM Chemicals
|a Lymphocyte Function-Associated Antigen-1
650 _ 7 |0 0
|2 NLM Chemicals
|a Receptors, Cell Surface
650 _ 7 |0 0
|2 NLM Chemicals
|a Recombinant Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Viral Matrix Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Viral Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a sars7a protein, SARS virus
650 _ 7 |0 126547-89-5
|2 NLM Chemicals
|a Intercellular Adhesion Molecule-1
650 _ 7 |0 37558-16-0
|2 NLM Chemicals
|a Phorbol 12,13-Dibutyrate
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a CD11a/CD18
653 2 0 |2 Author
|a ICAM
653 2 0 |2 Author
|a post-infective downregulation
653 2 0 |2 Author
|a receptor
653 2 0 |2 Author
|a T lymphocytes
653 2 0 |2 Author
|a viral entry
700 1 _ |a Willbold, D.
|b 1
|u FZJ
|0 P:(DE-Juel1)132029
773 _ _ |a 10.1515/BC.2007.157
|g Vol. 388, p. 1325 - 1332
|p 1325 - 1332
|q 388<1325 - 1332
|0 PERI:(DE-600)1466062-3
|t Biological chemistry
|v 388
|y 2007
|x 1431-6730
856 7 _ |u http://dx.doi.org/10.1515/BC.2007.157
856 4 _ |u https://juser.fz-juelich.de/record/60124/files/%5BBiological%20Chemistry%5D%20SARS-CoV%20accessory%20protein%207a%20directly%20interacts%20with%20human%20LFA-1.pdf
|y OpenAccess
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|x icon
|y OpenAccess
856 4 _ |u https://juser.fz-juelich.de/record/60124/files/%5BBiological%20Chemistry%5D%20SARS-CoV%20accessory%20protein%207a%20directly%20interacts%20with%20human%20LFA-1.jpg?subformat=icon-180
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856 4 _ |u https://juser.fz-juelich.de/record/60124/files/%5BBiological%20Chemistry%5D%20SARS-CoV%20accessory%20protein%207a%20directly%20interacts%20with%20human%20LFA-1.jpg?subformat=icon-700
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909 C O |o oai:juser.fz-juelich.de:60124
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913 1 _ |k P33
|v Funktion und Dysfunktion des Nervensystems
|l Funktion und Dysfunktion des Nervensystems
|b Gesundheit
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914 1 _ |y 2007
915 _ _ |a OpenAccess
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915 _ _ |a JCR/ISI refereed
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920 1 _ |k INB-2
|l Molekulare Biophysik
|d 31.12.2008
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