000060125 001__ 60125
000060125 005__ 20200423204551.0
000060125 0247_ $$2pmid$$apmid:17916189
000060125 0247_ $$2DOI$$a10.1111/j.1742-4658.2007.06073.x
000060125 0247_ $$2WOS$$aWOS:000250297600009
000060125 0247_ $$2Handle$$a2128/4688
000060125 0247_ $$2Handle$$a2128/4687
000060125 037__ $$aPreJuSER-60125
000060125 041__ $$aeng
000060125 082__ $$a540
000060125 084__ $$2WoS$$aBiochemistry & Molecular Biology
000060125 1001_ $$0P:(DE-Juel1)132012$$aMohrlüder, J.$$b0$$uFZJ
000060125 245__ $$aIdentification of calreticulin as ligand of GABARAP by phage display screening of a peptide library
000060125 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2007
000060125 300__ $$a5543 - 5555
000060125 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000060125 3367_ $$2DataCite$$aOutput Types/Journal article
000060125 3367_ $$00$$2EndNote$$aJournal Article
000060125 3367_ $$2BibTeX$$aARTICLE
000060125 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000060125 3367_ $$2DRIVER$$aarticle
000060125 440_0 $$017341$$aFEBS Journal$$v274$$x1742-464X
000060125 500__ $$aRecord converted from VDB: 12.11.2012
000060125 520__ $$a4-Aminobutyrate type A (GABA(A)) receptor-associated protein (GABARAP) is a ubiquitin-like modifier implicated in the intracellular trafficking of GABA(A) receptors, and belongs to a family of proteins involved in intracellular vesicular transport processes, such as autophagy and intra-Golgi transport. In this article, it is demonstrated that calreticulin is a high affinity ligand of GABARAP. Calreticulin, although best known for its functions as a Ca(2+) -dependent chaperone and a Ca(2+) -buffering protein in the endoplasmic reticulum, is also localized to the cytosol and exerts a variety of extra-endoplasmic reticulum functions. By phage display screening of a randomized peptide library, peptides that specifically bind GABARAP were identified. Their amino acid sequences allowed us to identify calreticulin as a potential GABARAP binding protein. GABARAP binding to calreticulin was confirmed by pull-down experiments with brain lysate and colocalization studies in N2a cells. Calreticulin and GABARAP interact with a dissociation constant K(d) = 64 nm and a mean lifetime of the complex of 20 min. Thus, the interaction between GABARAP and calreticulin is the strongest so far reported for each protein.
000060125 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000060125 588__ $$aDataset connected to Web of Science, Pubmed
000060125 65320 $$2Author$$acalreticulin
000060125 65320 $$2Author$$aGABA(A) receptor
000060125 65320 $$2Author$$aGABARAP
000060125 65320 $$2Author$$aphage display screening
000060125 65320 $$2Author$$aprotein-protein interaction
000060125 650_2 $$2MeSH$$aAmino Acid Motifs
000060125 650_2 $$2MeSH$$aAmino Acid Sequence
000060125 650_2 $$2MeSH$$aAnimals
000060125 650_2 $$2MeSH$$aBinding Sites
000060125 650_2 $$2MeSH$$aCalreticulin: chemistry
000060125 650_2 $$2MeSH$$aCalreticulin: metabolism
000060125 650_2 $$2MeSH$$aCells, Cultured
000060125 650_2 $$2MeSH$$aImmunohistochemistry
000060125 650_2 $$2MeSH$$aLigands
000060125 650_2 $$2MeSH$$aMicrotubule-Associated Proteins: chemistry
000060125 650_2 $$2MeSH$$aMicrotubule-Associated Proteins: metabolism
000060125 650_2 $$2MeSH$$aModels, Molecular
000060125 650_2 $$2MeSH$$aMolecular Sequence Data
000060125 650_2 $$2MeSH$$aPeptide Fragments: chemistry
000060125 650_2 $$2MeSH$$aPeptide Fragments: metabolism
000060125 650_2 $$2MeSH$$aPeptide Library
000060125 650_2 $$2MeSH$$aRats
000060125 650_2 $$2MeSH$$aSurface Plasmon Resonance
000060125 650_7 $$00$$2NLM Chemicals$$aCalreticulin
000060125 650_7 $$00$$2NLM Chemicals$$aGABARAP protein, rat
000060125 650_7 $$00$$2NLM Chemicals$$aLigands
000060125 650_7 $$00$$2NLM Chemicals$$aMicrotubule-Associated Proteins
000060125 650_7 $$00$$2NLM Chemicals$$aPeptide Fragments
000060125 650_7 $$00$$2NLM Chemicals$$aPeptide Library
000060125 650_7 $$2WoSType$$aJ
000060125 7001_ $$0P:(DE-Juel1)VDB8627$$aStangler, T.$$b1$$uFZJ
000060125 7001_ $$0P:(DE-Juel1)VDB15437$$aWiesehan, K.$$b2$$uFZJ
000060125 7001_ $$0P:(DE-Juel1)VDB65464$$aHoffmann, Y.$$b3$$uFZJ
000060125 7001_ $$0P:(DE-Juel1)VDB64411$$aMataruga, A.$$b4$$uFZJ
000060125 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b5$$uFZJ
000060125 773__ $$0PERI:(DE-600)2172518-4$$a10.1111/j.1742-4658.2007.06073.x$$gVol. 274, p. 5543 - 5555$$n21$$p5543 - 5555$$q274<5543 - 5555$$tThe @FEBS journal$$v274$$x1742-464X$$y2007
000060125 8567_ $$uhttp://dx.doi.org/10.1111/j.1742-4658.2007.06073.x
000060125 8564_ $$uhttps://juser.fz-juelich.de/record/60125/files/4688.pdf$$yOpenAccess
000060125 8564_ $$uhttps://juser.fz-juelich.de/record/60125/files/4688.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000060125 8564_ $$uhttps://juser.fz-juelich.de/record/60125/files/4688.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000060125 8564_ $$uhttps://juser.fz-juelich.de/record/60125/files/4688.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000060125 909CO $$ooai:juser.fz-juelich.de:60125$$pdnbdelivery$$pVDB$$pdriver$$popen_access$$popenaire
000060125 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000060125 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000060125 9141_ $$y2007
000060125 9131_ $$0G:(DE-Juel1)FUEK409$$bGesundheit$$kP33$$lFunktion und Dysfunktion des Nervensystems$$vFunktion und Dysfunktion des Nervensystems$$x0
000060125 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
000060125 9201_ $$0I:(DE-Juel1)VDB1045$$gJARA$$kJARA-SIM$$lJülich-Aachen Research Alliance - Simulation Sciences$$x1
000060125 970__ $$aVDB:(DE-Juel1)94325
000060125 9801_ $$aFullTexts
000060125 980__ $$aVDB
000060125 980__ $$aConvertedRecord
000060125 980__ $$ajournal
000060125 980__ $$aI:(DE-Juel1)ISB-2-20090406
000060125 980__ $$aI:(DE-Juel1)VDB1045
000060125 980__ $$aUNRESTRICTED
000060125 980__ $$aJUWEL
000060125 980__ $$aI:(DE-Juel1)ICS-6-20110106
000060125 980__ $$aFullTexts
000060125 981__ $$aI:(DE-Juel1)IBI-7-20200312
000060125 981__ $$aI:(DE-Juel1)ISB-2-20090406
000060125 981__ $$aI:(DE-Juel1)VDB1045
000060125 981__ $$aI:(DE-Juel1)ICS-6-20110106