TY  - JOUR
AU  - Mohrlüder, J.
AU  - Stangler, T.
AU  - Wiesehan, K.
AU  - Hoffmann, Y.
AU  - Mataruga, A.
AU  - Willbold, D.
TI  - Identification of calreticulin as ligand of GABARAP by phage display screening of a peptide library
JO  - The FEBS journal
VL  - 274
IS  - 21
SN  - 1742-464X
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-60125
SP  - 5543 - 5555
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - 4-Aminobutyrate type A (GABA(A)) receptor-associated protein (GABARAP) is a ubiquitin-like modifier implicated in the intracellular trafficking of GABA(A) receptors, and belongs to a family of proteins involved in intracellular vesicular transport processes, such as autophagy and intra-Golgi transport. In this article, it is demonstrated that calreticulin is a high affinity ligand of GABARAP. Calreticulin, although best known for its functions as a Ca(2+) -dependent chaperone and a Ca(2+) -buffering protein in the endoplasmic reticulum, is also localized to the cytosol and exerts a variety of extra-endoplasmic reticulum functions. By phage display screening of a randomized peptide library, peptides that specifically bind GABARAP were identified. Their amino acid sequences allowed us to identify calreticulin as a potential GABARAP binding protein. GABARAP binding to calreticulin was confirmed by pull-down experiments with brain lysate and colocalization studies in N2a cells. Calreticulin and GABARAP interact with a dissociation constant K(d) = 64 nm and a mean lifetime of the complex of 20 min. Thus, the interaction between GABARAP and calreticulin is the strongest so far reported for each protein.
KW  - Amino Acid Motifs
KW  - Amino Acid Sequence
KW  - Animals
KW  - Binding Sites
KW  - Calreticulin: chemistry
KW  - Calreticulin: metabolism
KW  - Cells, Cultured
KW  - Immunohistochemistry
KW  - Ligands
KW  - Microtubule-Associated Proteins: chemistry
KW  - Microtubule-Associated Proteins: metabolism
KW  - Models, Molecular
KW  - Molecular Sequence Data
KW  - Peptide Fragments: chemistry
KW  - Peptide Fragments: metabolism
KW  - Peptide Library
KW  - Rats
KW  - Surface Plasmon Resonance
KW  - Calreticulin (NLM Chemicals)
KW  - GABARAP protein, rat (NLM Chemicals)
KW  - Ligands (NLM Chemicals)
KW  - Microtubule-Associated Proteins (NLM Chemicals)
KW  - Peptide Fragments (NLM Chemicals)
KW  - Peptide Library (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17916189
UR  - <Go to ISI:>//WOS:000250297600009
DO  - DOI:10.1111/j.1742-4658.2007.06073.x
UR  - https://juser.fz-juelich.de/record/60125
ER  -