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000060128 0247_ $$2DOI$$a10.1016/j.bbamem.2007.10.023
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000060128 084__ $$2WoS$$aBiochemistry & Molecular Biology
000060128 084__ $$2WoS$$aBiophysics
000060128 1001_ $$0P:(DE-Juel1)VDB28257$$aWittlich, M.$$b0$$uFZJ
000060128 245__ $$aStructural characterisation of the transmembrane and cytoplasmic domains of human CD4
000060128 260__ $$aAmsterdam$$bElsevier$$c2007
000060128 300__ $$a2949 - 2960
000060128 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000060128 440_0 $$019423$$aBBA - Biomembranes$$v1768$$x0005-2736
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000060128 520__ $$aCluster determinant 4 (CD4) is a type I transmembrane glycoprotein of 58 kDa. It consists of an extracellular domain of 370 amino acids, a short transmembrane region, and a cytoplasmic domain of 40 amino acids at the C-terminal end. We investigated the structure of the 62 C-terminal residues of CD4, comprising its transmembrane and cytoplasmic domains. The five cysteine residues of this region have been replaced with serine and histidine residues in the polypeptide CD4mut. Uniformly 15N and 13C labeled protein was recombinantly expressed in E. coli and purified. Functional binding activity of CD4mut to protein VpU of the human immunodeficiency virus type 1 (HIV-1) was verified. Close to complete NMR resonance assignment of the 1H, 13C, and 15N spins of CD4mut was accomplished. The secondary structure of CD4mut in membrane simulating dodecylphosphocholine (DPC) micelles was characterized based on secondary chemical shift analysis, NOE-based proton-proton distances, and circular dichroism spectroscopy. A stable transmembrane helix and a short amphipathic helix in the cytoplasmic region were identified. The fractional helicity of the cytoplasmic helix appears to be stabilized in the presence of DPC micelles, although the extension of this helix is reduced in comparison to previous studies on synthetic peptides in aqueous solution. The role of the amphipathic helix and its potentially variable length is discussed with respect to the biological functions of CD4.
000060128 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000060128 588__ $$aDataset connected to Web of Science, Pubmed
000060128 650_2 $$2MeSH$$aAmino Acid Sequence
000060128 650_2 $$2MeSH$$aAntigens, CD4: chemistry
000060128 650_2 $$2MeSH$$aAntigens, CD4: genetics
000060128 650_2 $$2MeSH$$aAntigens, CD4: metabolism
000060128 650_2 $$2MeSH$$aCarbon Isotopes
000060128 650_2 $$2MeSH$$aCircular Dichroism
000060128 650_2 $$2MeSH$$aHuman Immunodeficiency Virus Proteins: metabolism
000060128 650_2 $$2MeSH$$aHumans
000060128 650_2 $$2MeSH$$aMagnetic Resonance Spectroscopy: methods
000060128 650_2 $$2MeSH$$aModels, Molecular
000060128 650_2 $$2MeSH$$aMolecular Sequence Data
000060128 650_2 $$2MeSH$$aMutation
000060128 650_2 $$2MeSH$$aNitrogen Isotopes
000060128 650_2 $$2MeSH$$aProtein Binding
000060128 650_2 $$2MeSH$$aProtein Structure, Secondary
000060128 650_2 $$2MeSH$$aProtein Structure, Tertiary
000060128 650_2 $$2MeSH$$aViral Regulatory and Accessory Proteins: metabolism
000060128 650_7 $$00$$2NLM Chemicals$$aAntigens, CD4
000060128 650_7 $$00$$2NLM Chemicals$$aCarbon Isotopes
000060128 650_7 $$00$$2NLM Chemicals$$aHuman Immunodeficiency Virus Proteins
000060128 650_7 $$00$$2NLM Chemicals$$aNitrogen Isotopes
000060128 650_7 $$00$$2NLM Chemicals$$aViral Regulatory and Accessory Proteins
000060128 650_7 $$2WoSType$$aJ
000060128 65320 $$2Author$$aCD4
000060128 65320 $$2Author$$aHIV-1
000060128 65320 $$2Author$$aVpU
000060128 65320 $$2Author$$amembrane protein
000060128 65320 $$2Author$$aNMR
000060128 65320 $$2Author$$aCD
000060128 7001_ $$0P:(DE-Juel1)132009$$aKoenig, B. W.$$b1$$uFZJ
000060128 7001_ $$0P:(DE-Juel1)VDB630$$aHoffmann, S.$$b2$$uFZJ
000060128 7001_ $$0P:(DE-Juel1)132029$$aWillbold, D.$$b3$$uFZJ
000060128 773__ $$0PERI:(DE-600)2209384-9$$a10.1016/j.bbamem.2007.10.023$$gVol. 1768, p. 2949 - 2960$$p2949 - 2960$$q1768<2949 - 2960$$tBiochimica et biophysica acta / Biomembranes$$v1768$$x0005-2736$$y2007
000060128 8567_ $$uhttp://dx.doi.org/10.1016/j.bbamem.2007.10.023
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000060128 9141_ $$y2007
000060128 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000060128 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
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