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000060251 0247_ $$2DOI$$a10.1016/j.bbapap.2007.09.005
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000060251 041__ $$aeng
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000060251 084__ $$2WoS$$aBiochemistry & Molecular Biology
000060251 084__ $$2WoS$$aBiophysics
000060251 1001_ $$0P:(DE-Juel1)VDB72839$$aStrucksberg, K.H.$$b0$$uFZJ
000060251 245__ $$aReversible and irreversible unfolding of multi-domain proteins
000060251 260__ $$aAmsterdam [u.a.]$$bElsevier$$c2007
000060251 300__ $$a1501 - 1603
000060251 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000060251 440_0 $$019421$$aBBA - Proteins and Proteomics$$v1774$$x1570-9639
000060251 500__ $$aRecord converted from VDB: 12.11.2012
000060251 520__ $$aIn contrast to single-domain proteins unfolding of larger multi-domain proteins is often irreversible. In a comparative case study on three different multi-domain proteins (phosphoglycerate kinase: PGK and two homologous alpha-amylases: TAKA and BLA) we investigated properties of unfolded states and their ability to fold back into the native state. For this purpose guanidine hydrochloride, alkaline pH, and thermal unfolded states were characterized. Structural alterations upon unfolding and refolding transitions were monitored using fluorescence and CD spectroscopy. Static and dynamic light scattering was employed to follow aggregation processes. Furthermore, proper refolding was also investigated by enzyme activity measurements. While for PGK at least partial reversible unfolding transitions were observed in most cases, we found reversible unfolding for TAKA in the case of alkaline pH and GndHCl induced unfolding. BLA exhibits reversible unfolding only under conditions with high concentrations of protecting osmolytes (glycerol), indicating that aggregation of the unfolded state is the main obstacle to achieve proper refolding for this protein. Structural properties, such as number and size of domains, secondary structure contents and compositions within domains, and domain topology were analyzed and considered in the interpretation of differences in refolding behavior of the investigated proteins.
000060251 536__ $$0G:(DE-Juel1)FUEK409$$2G:(DE-HGF)$$aFunktion und Dysfunktion des Nervensystems$$cP33$$x0
000060251 588__ $$aDataset connected to Web of Science, Pubmed
000060251 650_2 $$2MeSH$$aAspergillus oryzae: enzymology
000060251 650_2 $$2MeSH$$aBacillus: enzymology
000060251 650_2 $$2MeSH$$aBuffers
000060251 650_2 $$2MeSH$$aGlycerol: pharmacology
000060251 650_2 $$2MeSH$$aGuanidine: pharmacology
000060251 650_2 $$2MeSH$$aHydrogen-Ion Concentration
000060251 650_2 $$2MeSH$$aModels, Molecular
000060251 650_2 $$2MeSH$$aOsmolar Concentration
000060251 650_2 $$2MeSH$$aPhosphoglycerate Kinase: chemistry
000060251 650_2 $$2MeSH$$aProtein Denaturation: drug effects
000060251 650_2 $$2MeSH$$aProtein Folding
000060251 650_2 $$2MeSH$$aProtein Structure, Tertiary: drug effects
000060251 650_2 $$2MeSH$$aProtein Structure, Tertiary: physiology
000060251 650_2 $$2MeSH$$aTemperature
000060251 650_2 $$2MeSH$$aTransition Temperature
000060251 650_2 $$2MeSH$$aalpha-Amylases: chemistry
000060251 650_7 $$00$$2NLM Chemicals$$aBuffers
000060251 650_7 $$0113-00-8$$2NLM Chemicals$$aGuanidine
000060251 650_7 $$056-81-5$$2NLM Chemicals$$aGlycerol
000060251 650_7 $$0EC 2.7.2.3$$2NLM Chemicals$$aPhosphoglycerate Kinase
000060251 650_7 $$0EC 3.2.1.1$$2NLM Chemicals$$aalpha-Amylases
000060251 650_7 $$2WoSType$$aJ
000060251 65320 $$2Author$$aalpha-amylase
000060251 65320 $$2Author$$aaggregation
000060251 65320 $$2Author$$aunfolded states
000060251 65320 $$2Author$$aprotecting osmolyte
000060251 65320 $$2Author$$ahydrodynamic radius
000060251 7001_ $$0P:(DE-Juel1)VDB72840$$aRosenkranz, T.$$b1$$uFZJ
000060251 7001_ $$0P:(DE-Juel1)131961$$aFitter, J.$$b2$$uFZJ
000060251 773__ $$0PERI:(DE-600)2209540-8$$a10.1016/j.bbapap.2007.09.005$$gVol. 1774, p. 1501 - 1603$$p1501 - 1603$$q1774<1501 - 1603$$tBiochimica et biophysica acta / Proteins and proteomics$$v1774$$x1570-9639$$y2007
000060251 8567_ $$uhttp://dx.doi.org/10.1016/j.bbapap.2007.09.005
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000060251 9141_ $$y2007
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000060251 9201_ $$0I:(DE-Juel1)VDB805$$d31.12.2008$$gINB$$kINB-2$$lMolekulare Biophysik$$x0
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