%0 Journal Article
%A Mach, T.
%A Chimerel, C.
%A Fritz, J.
%A Fertig, N.
%A Winterhalter, M.
%A Fütterer, C.
%T Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion
%J Analytical and bioanalytical chemistry
%V 390
%@ 1618-2642
%C Berlin
%I Springer
%M PreJuSER-61424
%P 841 - 846
%D 2008
%Z Record converted from VDB: 12.11.2012
%X A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open head-stage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.
%K Cytoplasm: metabolism
%K Dimethylpolysiloxanes: chemistry
%K Electrochemistry: methods
%K Electrophysiology: instrumentation
%K Electrophysiology: methods
%K Equipment Design
%K Ions
%K Lipid Bilayers: chemistry
%K Liposomes: chemistry
%K Microfluidic Analytical Techniques
%K Miniaturization
%K Nanotechnology: methods
%K Patch-Clamp Techniques
%K Perfusion
%K Silicones: chemistry
%K Solvents: chemistry
%K Dimethylpolysiloxanes (NLM Chemicals)
%K Ions (NLM Chemicals)
%K Lipid Bilayers (NLM Chemicals)
%K Liposomes (NLM Chemicals)
%K Silicones (NLM Chemicals)
%K Solvents (NLM Chemicals)
%K baysilon (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:17972068
%U <Go to ISI:>//WOS:000252417800008
%R 10.1007/s00216-007-1647-7
%U https://juser.fz-juelich.de/record/61424