TY  - JOUR
AU  - Mach, T.
AU  - Chimerel, C.
AU  - Fritz, J.
AU  - Fertig, N.
AU  - Winterhalter, M.
AU  - Fütterer, C.
TI  - Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion
JO  - Analytical and bioanalytical chemistry
VL  - 390
SN  - 1618-2642
CY  - Berlin
PB  - Springer
M1  - PreJuSER-61424
SP  - 841 - 846
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open head-stage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.
KW  - Cytoplasm: metabolism
KW  - Dimethylpolysiloxanes: chemistry
KW  - Electrochemistry: methods
KW  - Electrophysiology: instrumentation
KW  - Electrophysiology: methods
KW  - Equipment Design
KW  - Ions
KW  - Lipid Bilayers: chemistry
KW  - Liposomes: chemistry
KW  - Microfluidic Analytical Techniques
KW  - Miniaturization
KW  - Nanotechnology: methods
KW  - Patch-Clamp Techniques
KW  - Perfusion
KW  - Silicones: chemistry
KW  - Solvents: chemistry
KW  - Dimethylpolysiloxanes (NLM Chemicals)
KW  - Ions (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Liposomes (NLM Chemicals)
KW  - Silicones (NLM Chemicals)
KW  - Solvents (NLM Chemicals)
KW  - baysilon (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17972068
UR  - <Go to ISI:>//WOS:000252417800008
DO  - DOI:10.1007/s00216-007-1647-7
UR  - https://juser.fz-juelich.de/record/61424
ER  -