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@ARTICLE{Fitri:61658,
      author       = {Fitri, N. and Kastenholz, B. and Buchari, B. and Amran, M.
                      B. and Warganegara, F. M.},
      title        = {{M}olybdenum speciation in raw phloem sap of castor bean},
      journal      = {Analytical letters},
      volume       = {41},
      issn         = {0003-2719},
      address      = {New York, NY},
      publisher    = {Taylor $\&$ Francis},
      reportid     = {PreJuSER-61658},
      pages        = {1773 - 1784},
      year         = {2008},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Separation and quantification of molybdenum (Mo) in raw
                      phloem sap from castor bean (Ricinus communis L.) was
                      performed by size exclusion chromatography (SEC) and further
                      purification was performed using quantitative preparative
                      native continuous polyacrylamide gel electrophoresis
                      (QPNC-PAGE). For elemental detection, an inductively coupled
                      plasma quadrupole mass spectrometer (ICP-QMS) was applied.
                      Two different SEC columns were utilized: column A, Sephadex
                      G-50 SF (700mm24mm), and column B, Sephadex G-25 M
                      (28mm9mm). The protein content of the fractions was
                      determined by the Bradford method. Using column A, two peaks
                      of Mo were detected consisting of a main peak (MoA2) in the
                      low molecular weight area (1.35 kDa), and a minor peak
                      (MoA1,30 kDa) at the void volume of the column. Both Mo
                      species were detected at the ultraviolet (UV) active
                      absorption area of raw phloem sap. Two peaks of Mo were also
                      detected using column B, the first peak (MoB1) being at the
                      same elution volume as the protein of raw phloem sap, and
                      the second one (MoB2) was eluted in the area of 1.5 to 2.4mL
                      of elution volume. Raw phloem sap digested by proteinase
                      K-enzyme indicates a significant reduction of MoB1 peak,
                      which suggests that the peak may contain Mo bound to protein
                      or polypeptides. The raw phloem sap and SEC fraction
                      containing highest Mo concentration (MoA2) were furthermore
                      separated by QPNC-PAGE. The result reveals that the
                      Mo-containing fraction from the raw phloem sap was eluted at
                      the same retention volume as the purified sample. This
                      implies that the Mo species were also successfully separated
                      and purified using QPNC-PAGE.},
      keywords     = {J (WoSType)},
      cin          = {ICG-3},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICG-3-20090406},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000258079500006},
      doi          = {10.1080/00032710802162442},
      url          = {https://juser.fz-juelich.de/record/61658},
}