Hauptseite > Publikationsdatenbank > Molybdenum speciation in raw phloem sap of castor bean > print

001     61658
005     20190625111504.0
024 7 _ |2 DOI
|a 10.1080/00032710802162442
024 7 _ |2 WOS
|a WOS:000258079500006
024 7 _ |a altmetric:3398899
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037 _ _ |a PreJuSER-61658
041 _ _ |a eng
082 _ _ |a 540
084 _ _ |2 WoS
|a Chemistry, Analytical
100 1 _ |a Fitri, N.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB71401
245 _ _ |a Molybdenum speciation in raw phloem sap of castor bean
260 _ _ |a New York, NY
|b Taylor & Francis
|c 2008
300 _ _ |a 1773 - 1784
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Analytical Letters
|x 0003-2719
|0 7961
|y 10
|v 41
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Separation and quantification of molybdenum (Mo) in raw phloem sap from castor bean (Ricinus communis L.) was performed by size exclusion chromatography (SEC) and further purification was performed using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE). For elemental detection, an inductively coupled plasma quadrupole mass spectrometer (ICP-QMS) was applied. Two different SEC columns were utilized: column A, Sephadex G-50 SF (700mm24mm), and column B, Sephadex G-25 M (28mm9mm). The protein content of the fractions was determined by the Bradford method. Using column A, two peaks of Mo were detected consisting of a main peak (MoA2) in the low molecular weight area (1.35 kDa), and a minor peak (MoA1,30 kDa) at the void volume of the column. Both Mo species were detected at the ultraviolet (UV) active absorption area of raw phloem sap. Two peaks of Mo were also detected using column B, the first peak (MoB1) being at the same elution volume as the protein of raw phloem sap, and the second one (MoB2) was eluted in the area of 1.5 to 2.4mL of elution volume. Raw phloem sap digested by proteinase K-enzyme indicates a significant reduction of MoB1 peak, which suggests that the peak may contain Mo bound to protein or polypeptides. The raw phloem sap and SEC fraction containing highest Mo concentration (MoA2) were furthermore separated by QPNC-PAGE. The result reveals that the Mo-containing fraction from the raw phloem sap was eluted at the same retention volume as the purified sample. This implies that the Mo species were also successfully separated and purified using QPNC-PAGE.
536 _ _ |a Terrestrische Umwelt
|c P24
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|0 G:(DE-Juel1)FUEK407
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588 _ _ |a Dataset connected to Web of Science
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a castor bean
653 2 0 |2 Author
|a Mo speciation
653 2 0 |2 Author
|a phloem sap
653 2 0 |2 Author
|a QPNC-PAGE
653 2 0 |2 Author
|a SEC
700 1 _ |a Kastenholz, B.
|b 1
|u FZJ
|0 P:(DE-Juel1)129343
700 1 _ |a Buchari, B.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Amran, M. B.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Warganegara, F. M.
|b 4
|0 P:(DE-HGF)0
773 _ _ |a 10.1080/00032710802162442
|g Vol. 41, p. 1773 - 1784
|p 1773 - 1784
|q 41<1773 - 1784
|0 PERI:(DE-600)2098207-0
|t Analytical letters
|v 41
|y 2008
|x 0003-2719
856 7 _ |u http://dx.doi.org/10.1080/00032710802162442
909 C O |o oai:juser.fz-juelich.de:61658
|p VDB
913 1 _ |k P24
|v Terrestrische Umwelt
|l Terrestrische Umwelt
|b Erde und Umwelt
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|x 0
914 1 _ |y 2008
915 _ _ |0 StatID:(DE-HGF)0010
|a JCR/ISI refereed
920 1 _ |k ICG-3
|l Phytosphäre
|d 31.10.2010
|g ICG
|0 I:(DE-Juel1)ICG-3-20090406
|x 1
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980 _ _ |a UNRESTRICTED
981 _ _ |a I:(DE-Juel1)IBG-2-20101118
981 _ _ |a I:(DE-Juel1)ICG-3-20090406

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