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@ARTICLE{Alt:61869,
      author       = {Alt, F. and Weber, G. and Messerschmidt, J. and von Bohlen,
                      A. and Kastenholz, B. and Günther, K.},
      title        = {{B}onding states of palladium in phytosystems : first
                      results for endive},
      journal      = {Analytical letters},
      volume       = {35},
      issn         = {0003-2719},
      address      = {New York, NY},
      publisher    = {Taylor $\&$ Francis},
      reportid     = {PreJuSER-61869},
      pages        = {1349 - 1359},
      year         = {2002},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {The binding of palladium to organic ligands was studied in
                      leaves from contaminated endive. The palladium-treated
                      plants contained 8.7 ng Pd/g referring to wet weight. After
                      a cell breakdown in buffer the resulting homogenate was
                      separated into supernatant and pellet by centrifugation and
                      the distribution of palladium was determined. The liquid
                      fraction contained $40\%$ of the total palladium and was
                      further separated by ultrafiltration. This technique
                      revealed that $23\%$ of total palladium was present as high
                      molecular weight species (>10 kDa). After concentration, the
                      high molecular weight fraction was further investigated by
                      gel permeation chromatography (GPC). By this method only one
                      elution range for palladium was observed. The size range of
                      the GPC used was about 20-8000 kDa for globular proteins.
                      The palladium elution maximum was detected in the range of
                      about 160 kDa. The determinations of palladium were
                      performed by total-reflection X-ray fluorescence after
                      palladium separation and preconcentration.},
      keywords     = {J (WoSType)},
      cin          = {ICG-III},
      ddc          = {540},
      cid          = {I:(DE-Juel1)VDB49},
      pnm          = {Chemie und Dynamik der Geo-Biosphäre},
      pid          = {G:(DE-Juel1)FUEK257},
      shelfmark    = {Chemistry, Analytical},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000177342300006},
      doi          = {10.1081/AL-12000671},
      url          = {https://juser.fz-juelich.de/record/61869},
}