TY  - JOUR
AU  - Nagel, K. A.
AU  - Kastenholz, B.
AU  - Jahnke, S.
AU  - van Dusschoten, D.
AU  - Aach, T.
AU  - Mühlich, M.
AU  - Truhn, D.
AU  - Scharr, H.
AU  - Terjung, St.
AU  - Walter, A.
AU  - Schurr, U.
TI  - Temperature responses of roots: impact on growth, root system architecture and implications for phenotyping
JO  - Functional plant biology
VL  - 36
SN  - 1445-4408
CY  - Collingwood, Victoria
PB  - CSIRO Publ.
M1  - PreJuSER-6244
SP  - 947 - 959
PY  - 2009
N1  - Record converted from VDB: 12.11.2012
AB  - Root phenotyping is a challenging task, mainly because of the hidden nature of this organ. Only recently, imaging technologies have become available that allow us to elucidate the dynamic establishment of root structure and function in the soil. In root tips, optical analysis of the relative elemental growth rates in root expansion zones of hydroponically-grown plants revealed that it is the maximum intensity of cellular growth processes rather than the length of the root growth zone that control the acclimation to dynamic changes in temperature. Acclimation of entire root systems was studied at high throughput in agar-filled Petri dishes. In the present study, optical analysis of root system architecture showed that low temperature induced smaller branching angles between primary and lateral roots, which caused a reduction in the volume that roots access at lower temperature. Simulation of temperature gradients similar to natural soil conditions led to differential responses in basal and apical parts of the root system, and significantly affected the entire root system. These results were supported by first data on the response of root structure and carbon transport to different root zone temperatures. These data were acquired by combined magnetic resonance imaging (MRI) and positron emission tomography ( PET). They indicate acclimation of root structure and geometry to temperature and preferential accumulation of carbon near the root tip at low root zone temperatures. Overall, this study demonstrated the value of combining different phenotyping technologies that analyse processes at different spatial and temporal scales. Only such an integrated approach allows us to connect differences between genotypes obtained in artificial high throughput conditions with specific characteristics relevant for field performance. Thus, novel routes may be opened up for improved plant breeding as well as for mechanistic understanding of root structure and function.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000271464600013
DO  - DOI:10.1071/FP09184
UR  - https://juser.fz-juelich.de/record/6244
ER  -