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000062860 084__ $$2WoS$$aBiochemical Research Methods
000062860 084__ $$2WoS$$aChemistry, Analytical
000062860 1001_ $$0P:(DE-Juel1)129410$$aThiele, B.$$b0$$uFZJ
000062860 245__ $$aAnalysis of amino acids without derivatization in barley extracts by LC-MS-MS
000062860 260__ $$aBerlin$$bSpringer$$c2008
000062860 300__ $$a2663 - 2672
000062860 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000062860 440_0 $$08664$$aAnalytical and Bioanalytical Chemistry$$v391$$x1618-2642$$y7
000062860 500__ $$aRecord converted from VDB: 12.11.2012
000062860 520__ $$aA method has been developed for quantification of 20 amino acids as well as 13 (15)N-labeled amino acids in barley plants. The amino acids were extracted from plant tissues using aqueous HCl-ethanol and directly analyzed without further purification. Analysis of the underivatized amino acids was performed by liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry (MS-MS) in the positive ESI mode. Separation was achieved on a strong cation exchange column (Luna 5micro SCX 100A) with 30 mM ammonium acetate in water (solvent A) and 5% acetic acid in water (solvent B). Quantification was accomplished using d (2)-Phe as an internal standard. Calibration curves were linear over the range 0.5-50 microM, and limits of detection were estimated to be 0.1-3.0 microM. The mass-spectrometric technique was employed to study the regulation of amino acid levels in barley plants grown at 15 degrees C uniform root temperature (RT) and 20-10 degrees C vertical RT gradient (RTG). The LC-MS-MS results demonstrated enhanced concentration of free amino acids in shoots at 20-10 degrees C RTG, while total free amino acid concentration in roots was similarly low for both RT treatments. (15)NO(3) (-) labeling experiments showed lower (15)N/(14)N ratios for Glu, Ser, Ala and Val in plants grown at 20-10 degrees C RTG compared with those grown at 15 degrees C RT.
000062860 536__ $$0G:(DE-Juel1)FUEK407$$2G:(DE-HGF)$$aTerrestrische Umwelt$$cP24$$x0
000062860 588__ $$aDataset connected to Web of Science, Pubmed
000062860 650_2 $$2MeSH$$aAmino Acids: analysis
000062860 650_2 $$2MeSH$$aCalibration
000062860 650_2 $$2MeSH$$aCations: chemistry
000062860 650_2 $$2MeSH$$aChromatography, Ion Exchange: methods
000062860 650_2 $$2MeSH$$aHordeum: chemistry
000062860 650_2 $$2MeSH$$aHordeum: metabolism
000062860 650_2 $$2MeSH$$aNitrogen Isotopes
000062860 650_2 $$2MeSH$$aPlant Extracts: analysis
000062860 650_2 $$2MeSH$$aPlant Leaves: chemistry
000062860 650_2 $$2MeSH$$aPlant Leaves: metabolism
000062860 650_2 $$2MeSH$$aPlant Roots: chemistry
000062860 650_2 $$2MeSH$$aPlant Roots: metabolism
000062860 650_2 $$2MeSH$$aPlant Shoots: chemistry
000062860 650_2 $$2MeSH$$aPlant Shoots: metabolism
000062860 650_2 $$2MeSH$$aReproducibility of Results
000062860 650_2 $$2MeSH$$aSpectrometry, Mass, Electrospray Ionization: methods
000062860 650_2 $$2MeSH$$aTandem Mass Spectrometry: methods
000062860 650_7 $$00$$2NLM Chemicals$$aAmino Acids
000062860 650_7 $$00$$2NLM Chemicals$$aCations
000062860 650_7 $$00$$2NLM Chemicals$$aNitrogen Isotopes
000062860 650_7 $$00$$2NLM Chemicals$$aPlant Extracts
000062860 650_7 $$2WoSType$$aJ
000062860 65320 $$2Author$$aliquid chromatography-mass spectrometry
000062860 65320 $$2Author$$aamino acids
000062860 65320 $$2Author$$astrong cation exchange column
000062860 65320 $$2Author$$aplant metabolism
000062860 7001_ $$0P:(DE-Juel1)VDB28681$$aFüllner, K.$$b1$$uFZJ
000062860 7001_ $$0P:(DE-Juel1)VDB59598$$aStein, N.$$b2$$uFZJ
000062860 7001_ $$0P:(DE-Juel1)129053$$aOldiges, M.$$b3$$uFZJ
000062860 7001_ $$0P:(DE-Juel1)129349$$aKuhn, A. J.$$b4$$uFZJ
000062860 7001_ $$0P:(DE-Juel1)129471$$aHofmann, D.$$b5$$uFZJ
000062860 773__ $$0PERI:(DE-600)1459122-4$$a10.1007/s00216-008-2167-9$$gVol. 391, p. 2663 - 2672$$p2663 - 2672$$q391<2663 - 2672$$tAnalytical and bioanalytical chemistry$$v391$$x1618-2642$$y2008
000062860 8567_ $$uhttp://dx.doi.org/10.1007/s00216-008-2167-9
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