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| 001 | 62860 | ||
| 005 | 20190625111606.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:18506428 |
| 024 | 7 | _ | |2 DOI |a 10.1007/s00216-008-2167-9 |
| 024 | 7 | _ | |2 WOS |a WOS:000258083900030 |
| 024 | 7 | _ | |a altmetric:21819145 |2 altmetric |
| 037 | _ | _ | |a PreJuSER-62860 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 540 |
| 084 | _ | _ | |2 WoS |a Biochemical Research Methods |
| 084 | _ | _ | |2 WoS |a Chemistry, Analytical |
| 100 | 1 | _ | |a Thiele, B. |b 0 |u FZJ |0 P:(DE-Juel1)129410 |
| 245 | _ | _ | |a Analysis of amino acids without derivatization in barley extracts by LC-MS-MS |
| 260 | _ | _ | |a Berlin |b Springer |c 2008 |
| 300 | _ | _ | |a 2663 - 2672 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Analytical and Bioanalytical Chemistry |x 1618-2642 |0 8664 |y 7 |v 391 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a A method has been developed for quantification of 20 amino acids as well as 13 (15)N-labeled amino acids in barley plants. The amino acids were extracted from plant tissues using aqueous HCl-ethanol and directly analyzed without further purification. Analysis of the underivatized amino acids was performed by liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry (MS-MS) in the positive ESI mode. Separation was achieved on a strong cation exchange column (Luna 5micro SCX 100A) with 30 mM ammonium acetate in water (solvent A) and 5% acetic acid in water (solvent B). Quantification was accomplished using d (2)-Phe as an internal standard. Calibration curves were linear over the range 0.5-50 microM, and limits of detection were estimated to be 0.1-3.0 microM. The mass-spectrometric technique was employed to study the regulation of amino acid levels in barley plants grown at 15 degrees C uniform root temperature (RT) and 20-10 degrees C vertical RT gradient (RTG). The LC-MS-MS results demonstrated enhanced concentration of free amino acids in shoots at 20-10 degrees C RTG, while total free amino acid concentration in roots was similarly low for both RT treatments. (15)NO(3) (-) labeling experiments showed lower (15)N/(14)N ratios for Glu, Ser, Ala and Val in plants grown at 20-10 degrees C RTG compared with those grown at 15 degrees C RT. |
| 536 | _ | _ | |a Terrestrische Umwelt |c P24 |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK407 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Amino Acids: analysis |
| 650 | _ | 2 | |2 MeSH |a Calibration |
| 650 | _ | 2 | |2 MeSH |a Cations: chemistry |
| 650 | _ | 2 | |2 MeSH |a Chromatography, Ion Exchange: methods |
| 650 | _ | 2 | |2 MeSH |a Hordeum: chemistry |
| 650 | _ | 2 | |2 MeSH |a Hordeum: metabolism |
| 650 | _ | 2 | |2 MeSH |a Nitrogen Isotopes |
| 650 | _ | 2 | |2 MeSH |a Plant Extracts: analysis |
| 650 | _ | 2 | |2 MeSH |a Plant Leaves: chemistry |
| 650 | _ | 2 | |2 MeSH |a Plant Leaves: metabolism |
| 650 | _ | 2 | |2 MeSH |a Plant Roots: chemistry |
| 650 | _ | 2 | |2 MeSH |a Plant Roots: metabolism |
| 650 | _ | 2 | |2 MeSH |a Plant Shoots: chemistry |
| 650 | _ | 2 | |2 MeSH |a Plant Shoots: metabolism |
| 650 | _ | 2 | |2 MeSH |a Reproducibility of Results |
| 650 | _ | 2 | |2 MeSH |a Spectrometry, Mass, Electrospray Ionization: methods |
| 650 | _ | 2 | |2 MeSH |a Tandem Mass Spectrometry: methods |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Amino Acids |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Cations |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Nitrogen Isotopes |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Plant Extracts |
| 650 | _ | 7 | |a J |2 WoSType |
| 653 | 2 | 0 | |2 Author |a liquid chromatography-mass spectrometry |
| 653 | 2 | 0 | |2 Author |a amino acids |
| 653 | 2 | 0 | |2 Author |a strong cation exchange column |
| 653 | 2 | 0 | |2 Author |a plant metabolism |
| 700 | 1 | _ | |a Füllner, K. |b 1 |u FZJ |0 P:(DE-Juel1)VDB28681 |
| 700 | 1 | _ | |a Stein, N. |b 2 |u FZJ |0 P:(DE-Juel1)VDB59598 |
| 700 | 1 | _ | |a Oldiges, M. |b 3 |u FZJ |0 P:(DE-Juel1)129053 |
| 700 | 1 | _ | |a Kuhn, A. J. |b 4 |u FZJ |0 P:(DE-Juel1)129349 |
| 700 | 1 | _ | |a Hofmann, D. |b 5 |u FZJ |0 P:(DE-Juel1)129471 |
| 773 | _ | _ | |a 10.1007/s00216-008-2167-9 |g Vol. 391, p. 2663 - 2672 |p 2663 - 2672 |q 391<2663 - 2672 |0 PERI:(DE-600)1459122-4 |t Analytical and bioanalytical chemistry |v 391 |y 2008 |x 1618-2642 |
| 856 | 7 | _ | |u http://dx.doi.org/10.1007/s00216-008-2167-9 |
| 909 | C | O | |o oai:juser.fz-juelich.de:62860 |p VDB |
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| 914 | 1 | _ | |y 2008 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
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| 920 | 1 | _ | |k ICG-3 |l Phytosphäre |d 31.10.2010 |g ICG |0 I:(DE-Juel1)ICG-3-20090406 |x 1 |
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