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000062887 0247_ $$2DOI$$a10.1016/j.biomaterials.2008.06.020
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000062887 041__ $$aeng
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000062887 084__ $$2WoS$$aEngineering, Biomedical
000062887 084__ $$2WoS$$aMaterials Science, Biomaterials
000062887 1001_ $$0P:(DE-Juel1)VDB46736$$aSommerhage, F.$$b0$$uFZJ
000062887 245__ $$aMembrane allocation profiling: A method to characterize three-dimensional cell shape and attachment based on surface reconstruction
000062887 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2008
000062887 300__ $$a3927 - 3935
000062887 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000062887 440_0 $$0863$$aBiomaterials$$v29$$x0142-9612
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000062887 520__ $$aThree-dimensional surface reconstructions from high resolution image stacks of biological specimens, observed by confocal microscopy, have changed the perspective of morphological understanding. In the field of cell-cell or cell-substrate interfaces, combining these two techniques leads to new insights yet also creates a tremendous amount of data. In this article, we present a technique to reduce large, multidimensional data sets from confocal microscopy into one single curve: a membrane allocation profile. Reconstructed cells are represented in a three-dimensional surface from image sections of individual cells. We virtually cut segments of the reconstructed cell membrane parallel to the substrate and calculate the surface areas of each segment. The obtained membrane allocation profiles lead to morphological insights and yield an in vivo ratio of attached and free membrane areas without cell fixation. As an example, glass substrates were modified with different proteins (fibronectin, laminin, concavalin A, extracellular matrix gel, and both isomers of poly-lysine) and presented to HEK293 cells to examine differences in cell morphology and adhesion. We proved that proteins on a substrate could increase the attached portion of a cell membrane, facing the modified substrate, from an average of 32% (glass) to 45% (poly-lysine) of the total membrane surface area.
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000062887 650_2 $$2MeSH$$aCell Adhesion
000062887 650_2 $$2MeSH$$aCell Line
000062887 650_2 $$2MeSH$$aCell Membrane: chemistry
000062887 650_2 $$2MeSH$$aCell Membrane: metabolism
000062887 650_2 $$2MeSH$$aCell Shape
000062887 650_2 $$2MeSH$$aExtracellular Matrix: chemistry
000062887 650_2 $$2MeSH$$aHumans
000062887 650_2 $$2MeSH$$aImage Processing, Computer-Assisted
000062887 650_2 $$2MeSH$$aImaging, Three-Dimensional: instrumentation
000062887 650_2 $$2MeSH$$aImaging, Three-Dimensional: methods
000062887 650_2 $$2MeSH$$aSurface Properties
000062887 650_7 $$2WoSType$$aJ
000062887 65320 $$2Author$$acell adhesion
000062887 65320 $$2Author$$acell morphology
000062887 65320 $$2Author$$aconfocal microscopy
000062887 65320 $$2Author$$amembrane
000062887 65320 $$2Author$$asurface analysis
000062887 65320 $$2Author$$asurface modification
000062887 7001_ $$0P:(DE-Juel1)VDB56782$$aHelpenstein, R.$$b1$$uFZJ
000062887 7001_ $$0P:(DE-Juel1)VDB70820$$aRauf, A.$$b2$$uFZJ
000062887 7001_ $$0P:(DE-Juel1)VDB17030$$aWrobel, G.$$b3$$uFZJ
000062887 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, A.$$b4$$uFZJ
000062887 7001_ $$0P:(DE-Juel1)VDB5728$$aIngebrandt, S.$$b5$$uFZJ
000062887 773__ $$0PERI:(DE-600)2004010-6$$a10.1016/j.biomaterials.2008.06.020$$gVol. 29, p. 3927 - 3935$$p3927 - 3935$$q29<3927 - 3935$$tBiomaterials$$v29$$x0142-9612$$y2008
000062887 8567_ $$uhttp://dx.doi.org/10.1016/j.biomaterials.2008.06.020
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000062887 9141_ $$y2008
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000062887 9201_ $$0I:(DE-Juel1)IBN-2-20090406$$d31.12.2010$$gIBN$$kIBN-2$$lBioelektronik$$x0
000062887 9201_ $$0I:(DE-Juel1)VDB381$$d14.09.2008$$gCNI$$kCNI$$lCenter of Nanoelectronic Systems for Information Technology$$x1$$z381
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