TY  - JOUR
AU  - Sommerhage, F.
AU  - Helpenstein, R.
AU  - Rauf, A.
AU  - Wrobel, G.
AU  - Offenhäusser, A.
AU  - Ingebrandt, S.
TI  - Membrane allocation profiling: A method to characterize three-dimensional cell shape and attachment based on surface reconstruction
JO  - Biomaterials
VL  - 29
SN  - 0142-9612
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-62887
SP  - 3927 - 3935
PY  - 2008
N1  - Record converted from VDB: 12.11.2012
AB  - Three-dimensional surface reconstructions from high resolution image stacks of biological specimens, observed by confocal microscopy, have changed the perspective of morphological understanding. In the field of cell-cell or cell-substrate interfaces, combining these two techniques leads to new insights yet also creates a tremendous amount of data. In this article, we present a technique to reduce large, multidimensional data sets from confocal microscopy into one single curve: a membrane allocation profile. Reconstructed cells are represented in a three-dimensional surface from image sections of individual cells. We virtually cut segments of the reconstructed cell membrane parallel to the substrate and calculate the surface areas of each segment. The obtained membrane allocation profiles lead to morphological insights and yield an in vivo ratio of attached and free membrane areas without cell fixation. As an example, glass substrates were modified with different proteins (fibronectin, laminin, concavalin A, extracellular matrix gel, and both isomers of poly-lysine) and presented to HEK293 cells to examine differences in cell morphology and adhesion. We proved that proteins on a substrate could increase the attached portion of a cell membrane, facing the modified substrate, from an average of 32% (glass) to 45% (poly-lysine) of the total membrane surface area.
KW  - Cell Adhesion
KW  - Cell Line
KW  - Cell Membrane: chemistry
KW  - Cell Membrane: metabolism
KW  - Cell Shape
KW  - Extracellular Matrix: chemistry
KW  - Humans
KW  - Image Processing, Computer-Assisted
KW  - Imaging, Three-Dimensional: instrumentation
KW  - Imaging, Three-Dimensional: methods
KW  - Surface Properties
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:18621415
UR  - <Go to ISI:>//WOS:000259059100003
DO  - DOI:10.1016/j.biomaterials.2008.06.020
UR  - https://juser.fz-juelich.de/record/62887
ER  -