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@ARTICLE{Sommerhage:62887,
      author       = {Sommerhage, F. and Helpenstein, R. and Rauf, A. and Wrobel,
                      G. and Offenhäusser, A. and Ingebrandt, S.},
      title        = {{M}embrane allocation profiling: {A} method to characterize
                      three-dimensional cell shape and attachment based on surface
                      reconstruction},
      journal      = {Biomaterials},
      volume       = {29},
      issn         = {0142-9612},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {PreJuSER-62887},
      pages        = {3927 - 3935},
      year         = {2008},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Three-dimensional surface reconstructions from high
                      resolution image stacks of biological specimens, observed by
                      confocal microscopy, have changed the perspective of
                      morphological understanding. In the field of cell-cell or
                      cell-substrate interfaces, combining these two techniques
                      leads to new insights yet also creates a tremendous amount
                      of data. In this article, we present a technique to reduce
                      large, multidimensional data sets from confocal microscopy
                      into one single curve: a membrane allocation profile.
                      Reconstructed cells are represented in a three-dimensional
                      surface from image sections of individual cells. We
                      virtually cut segments of the reconstructed cell membrane
                      parallel to the substrate and calculate the surface areas of
                      each segment. The obtained membrane allocation profiles lead
                      to morphological insights and yield an in vivo ratio of
                      attached and free membrane areas without cell fixation. As
                      an example, glass substrates were modified with different
                      proteins (fibronectin, laminin, concavalin A, extracellular
                      matrix gel, and both isomers of poly-lysine) and presented
                      to HEK293 cells to examine differences in cell morphology
                      and adhesion. We proved that proteins on a substrate could
                      increase the attached portion of a cell membrane, facing the
                      modified substrate, from an average of $32\%$ (glass) to
                      $45\%$ (poly-lysine) of the total membrane surface area.},
      keywords     = {Cell Adhesion / Cell Line / Cell Membrane: chemistry / Cell
                      Membrane: metabolism / Cell Shape / Extracellular Matrix:
                      chemistry / Humans / Image Processing, Computer-Assisted /
                      Imaging, Three-Dimensional: instrumentation / Imaging,
                      Three-Dimensional: methods / Surface Properties / J
                      (WoSType)},
      cin          = {IBN-2 / CNI},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBN-2-20090406 / I:(DE-Juel1)VDB381},
      pnm          = {Grundlagen für zukünftige Informationstechnologien},
      pid          = {G:(DE-Juel1)FUEK412},
      shelfmark    = {Engineering, Biomedical / Materials Science, Biomaterials},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:18621415},
      UT           = {WOS:000259059100003},
      doi          = {10.1016/j.biomaterials.2008.06.020},
      url          = {https://juser.fz-juelich.de/record/62887},
}